This product provides fast and easy methods for the purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomski, plasmid) can be purified from bacterial cultures.
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation total DNA from bacterial culture |
| Prijave | PCR, southern blot and enzyme digestion, itd. |
| Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
| Procesna metoda | Ručno ili automatski |
| Vrsta uzorka | Gram negative or positive bacteria |
| Količina uzorka | Bacterial culture: 0.5-1.5ml |
| Volumen eluacije | ≥60μl |
| Vrijeme po vožnji | ≤60 minuta |
Načelo
- This product utilizes high-binding magnetic particles for purification.
- Samples are lysed and digested using a lysate and Lysozyme.
- DNA is released into the lysate during this process.
- Magnetic particles and binding solution are added, allowing DNA to be adsorbed onto the surface of the particles while impurities such as proteins are not adsorbed.
- The adsorbed particles undergo washing with a washing solution to remove proteins and impurities.
- Ethanol is used for further washing to remove salts.
- Finally, DNA is eluted using Elution Buffer.
Prednosti
- Brzo: Multiple samples can be extracted within 60 minuta (after digestion), making the process time-efficient.
- Visoka čistoća: Unique adsorbents ensure the complete removal of inhibitory factors, resulting in high-purity DNA extraction.
- Visoki oporavak: DNA can be recovered at the level of picograms (PG), indicating a high level of efficiency in the extraction process.
Sadržaj kompleta
| Sadržaj | D513101 | D513102 | D513103 |
| Vremena pročišćavanja | 48 Pripreme | 96 Pripreme | 480 Pripreme |
| MagPure Particles | 1.2 ml | 2.5 ml | 11 ml |
| Lysozyme | 60 mg | 120 mg | 600 mg |
| Proteinaza K | 24 mg | 50 mg | 220 mg |
| Pufer za otapanje proteaze | 3 ml | 6 ml | 30 ml |
| Buffer SDS | 1.5 ml | 3 ml | 15 ml |
| Buffer MLA | 30 ml | 60 ml | 300 ml |
| Međuspremnik GW1* | 13 ml | 44 ml | 110 ml |
| Buffer TE | 30 ml | 40 ml | 200 ml |
Skladištenje i stabilnost
- MagPure Particles, Lysozyme, and Proteinase K should be stored at 2-8°C upon arrival.
- Short-term storage (do 12 tjedni) na sobnoj temperaturi (15-25°C) ne utječe na njihovu izvedbu.
- Preostale komponente kita mogu se čuvati na suhom na sobnoj temperaturi (15-25°C) and remain stable for at least 18 mjeseci pod ovim uvjetima.
Recenzije
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