Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation of total DNA from 200μl whole blood |
| Prijave | PCR, Southern Bolt i otkrivanje virusa, itd |
| Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
| Procesna metoda | Ručno ili automatski |
| Vrsta uzorka | Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells |
| Količina uzorka | 200μl |
| Volumen eluacije | ≥50μl |
| Vrijeme po vožnji | ≤60 minuta |
Načelo
This product is based on the purification method of high-binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNK se oslobađa u lizat. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with a washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally, DNA was eluted by an Elution Buffer.
Prednosti
- High binding force – suitable for handling DNA-rich samples, such as whole blood, buffy coat, concentrated blood, itd.
- Brzo – polydisperse magnetic beads, fast magnetic response, and short extraction time
- Visoka čistoća – the obtained DNA can be directly used for second-generation sequencing, PCR-based detection, gene bank, itd.
- Automatic – saving time and labor and safer
Sadržaj kompleta
| Sadržaj | D631101 | D631102 | D631103 |
| Vremena pročišćavanja | 48 | 96 | 480 |
| MagPure Particles | 1.2 ml | 2.5 ml | 11 ml |
| Proteinaza K | 24 mg | 50 mg | 220 mg |
| Pufer za otapanje proteaze | 1.8 ml | 5 ml | 15 ml |
| Buffer AL | 15 ml | 30 ml | 120 ml |
| Međuspremnik GW1* | 22 ml | 53 ml | 220 ml |
| Elucijski pufer | 15 ml | 30 ml | 100 ml |
Skladištenje i stabilnost
Proteinaza K, MagPure Particles should be stored at 2-8°C upon arrival. Međutim, kratkotrajno skladištenje (do 24 tjedni) na sobnoj temperaturi (15-25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na suhom na sobnoj temperaturi (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima. Cijeli komplet može se čuvati na 2-8°C, but in this case, buffers should be redissolved before use. Provjerite jesu li svi puferi na sobnoj temperaturi kada se koriste.
