This kit is designed to rich and extract 100bp-500bp circulating DNA from 5 ml cell-free body fluids (such asplasma, serum), and remove fragments above 500bp. Machine reaction only takes 90 minuta. Magnetic-particle technology provides high-quality DNA that is suitable for direct use in downstream applications such as PCR and next generation sequencing.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation circulating DNA from 5ml plasma, serum, body fluids |
| Prijave | qPCR, NGS, itd. |
| Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Serum, plasma |
| Količina uzorka | 5ml |
| Volumen eluacije | ≥40μl |
| Vrijeme po vožnji | ≤50 minuta |
Načelo
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNK se oslobađa u lizat. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.
Prednosti
- Economy – less than 50% of the price of Qiagen and other imported products
- Automatic – without labour
Sadržaj kompleta
| Sadržaj | 1292750 | 12927200 |
| Vremena pročišćavanja | 50 | 200 |
| MagPure Particles G | 20 ml | 80 ml |
| MagBind Particles (selection particles) | 14 ml | 58 ml |
| Selection Solution | 100 ml | 400 ml |
| Proteinaza K | 300 mg | 1.2 g |
| Pufer za otapanje proteaze | 25 ml | 100 ml |
| Buffer SDS(20%) | 15 ml | 60 ml |
| Buffer MLK | 300 ml | 3 x 450 ml |
| Buffer BST1 | 225 ml | 2x 450 ml |
| Buffer MKW1 | 225 ml | 2x 450 ml |
| Buffer MW2* | 50 ml | 2x 100 ml |
| Međuspremnik AE | 10 ml | 30 ml |
Skladištenje i stabilnost
MagPure Particles G, MagBind Particles and Proteinase K should bestored at 2–8°C upon arrival. Međutim, kratkotrajno skladištenje (do 12 tjedni) na sobnoj temperaturi (15–25°C) ne utječe na njihovu izvedbu. The remaining kit components can be stored dry at roomtemperature (15–25°C) i stabilni su najmanje 18 months underthese conditions.The entire kit can be stored at 2–8°C, ali u ovom slučaju pufere treba ponovno otopiti prije upotrebe. Provjerite jesu li svi puferi na sobnoj temperaturi kada se koriste.
Recenzije
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