Uvod
This product is suitable for rapid extraction of DNA from tissue, cells, krv, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
pojedinosti
Tehnički podaci
Značajke | Tehnički podaci |
Glavne funkcije | Isolation total DNA from 1-100ul blood, FFPE, tissue and other samples |
Prijave | Second generation sequencing, PCR, real timePCR, itd. |
Metoda pročišćavanja | Monodisperse magnetic beads |
Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
Procesna metoda | Ručno ili automatski |
Vrsta uzorka | Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells |
Količina uzorka | Body fluid : 10 – 200μl, Tkivo : <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≤10mg |
Prinos | 10ng – 15μg |
Volumen eluacije | 80 -100μl |
Vrijeme po vožnji |
Načelo
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNK se oslobađa u lizat. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Prednosti
- High binding force – suitable for handling DNA rich samples, such as whole blood, buffy coat, concentrated blood, itd.
- Visoka čistoća – carboxyl magnetic beads with weak surface adsorption, getting higher purity
- Općenito – can be used for various clinical samples
Sadržaj kompleta
Sadržaj | IVD3101 |
Vremena pročišćavanja | 200 |
MagBind Particles | 4.5 ml |
Proteinaza K | 90 mg |
Pufer za otapanje proteaze | 10 ml |
Buffer ATL | 60 ml |
Buffer AL | 60 ml |
Buffer BD* | 20 ml |
Buffer BXW1* | 110 ml |
Elucijski pufer | 30 ml |
Skladištenje i stabilnost
Proteinaza K, MagBind Particles should be stored at 2–8°C upon arrival. Međutim, short-termstorage (do 18 tjedni) na sobnoj temperaturi (15–25°C) ne utječe na njihovu izvedbu. The remainingkit components can be stored dry at room temperature (15–25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
Recenzije
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