Uvod

This product is suitable for extracting total pathogen nucleic acid from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, itd. The purified DNA/RNA can be used for clinical in vitro detection.

pojedinosti

Tehnički podaci

Načelo

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.

Prednosti

• Brzo – može se izdvojiti nekoliko uzoraka 40 minutes by column method
• High quality – high purity total RNA / DNA can be directly used in various sensitive downstream applications
• Sef – no phenol chloroform extraction required
• Sensitive – DNA/RNA can be recovered at the level of PG

Sadržaj kompleta

Skladištenje i stabilnost

MagPure Particles and Proteinase K should be stored at 2–8°C upon arrival. Međutim, kratkotrajno skladištenje (do 8 tjedni) na sobnoj temperaturi (15–25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15–25°C) and are stable for 18 mjeseci pod ovim uvjetima.