Uvod
The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation up to 15μg endotoxin free plasmid DNA from 1-5ml bacterial culture |
| Prijave | Enzyme digestion, sequencing, PCR and labeling, itd. |
| Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
| Procesna metoda | Ručno ili automatski |
| Vrsta uzorka | Konvencionalni plazmid, plasmid≤30KB |
| Količina uzorka | 1-5ml |
| Volumen eluacije | ≥50μl |
| Vrijeme po vožnji | ≤80 minutes |
Načelo
This product is based on the purification method of high-binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNK se oslobađa u lizat. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with a washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally, DNA was eluted with an Elution Buffer.
Prednosti
- Visoka čistoća – pročišćeni plazmid može se izravno koristiti u sekvenciranju, enzyme digestion PCR, itd.
- Brzo – it takes only 80 minutes to complete the isolation
- Visok prinos – up to 15μg plasmid can be bound in one column
- Economic – excellent cost-effectiveness performance
Sadržaj kompleta
| Sadržaj | P181402 | P181403 | P181404 |
| Vremena pročišćavanja | 100 Pripreme | 500 Pripreme | 5000 Pripreme |
| RNaza A | 10 mg | 30 mg | 2 x 160 mg |
| Buffer P1 | 30 ml | 150 ml | 2 x 800 ml |
| Buffer P2 | 30 ml | 150 ml | 2 x 800 ml |
| Buffer LEN3 | 20 ml | 80 ml | 800 ml |
| Buffer LN4 | 90 ml | 400 ml | 4 x 980 ml |
| MagPure Particles | 3.5 ml | 17 ml | 3 x 60 ml |
Skladištenje i stabilnost
RNase A and MagPure Particles should be stored at 2–8°C upon arrival. Međutim, kratkotrajno skladištenje (do 12 tjedni) na sobnoj temperaturi (15–25°C) ne utječe na njegovu izvedbu. Preostale komponente kita mogu se čuvati na suhom na sobnoj temperaturi (15–25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima. Cijeli komplet može se čuvati na 2–8°C, but in this case, buffers should be redissolved before use. Provjerite jesu li svi puferi na sobnoj temperaturi kada se koriste. Ako se u puferima stvori bilo kakav talog, zagrijte na 37 ℃ da se otopi. After the addition of RNase A, Međuspremnik P1 je stabilan za 6 months when stored at
Recenzije
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