Uvod
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
pojedinosti
Tehnički podaci
Značajke | Tehnički podaci |
Glavne funkcije | Extract viral DNA/RNA from200μl samples by magnetic beads |
Prijave | RT-PCR,PCR,NGS |
Proizvodi | Viral DNA / RNA, body cell DNA / RNA |
Metoda pročišćavanja | Polydisperse magnetic beads |
Tehnologija pročišćavanja | Magnetic beads technolog |
Procesna metoda | Ručno ili automatski |
Vrsta uzorka | |
Količina uzorka | 200μl |
Adaptive instrument | Nucleic acid extractor, pipetting workstation |
Prinos | |
Volumen eluacije | |
Vrijeme po vožnji | |
Nosivi volumen tekućine po stupcu | |
Prinos vezivanja kolone |
Načelo
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.
Prednosti
- Brzo – može se izdvojiti nekoliko uzoraka 40 minutes by column method
- High quality – high purity total RNA / DNA can be directly used in various sensitive downstream applications
- Sef – no phenol chloroform extraction required
- Sensitive – DNA/RNA can be recovered at the level of PG
Sadržaj kompleta
Sadržaj | IVD5412 |
Vremena pročišćavanja | 200 Pripreme |
MagPure Particles N | 5 ml |
PK/Carrier RNA | 50 mg |
Protease Dissolve Buffer Blue | 5 ml |
Buffer MLB | 120 ml |
Buffer MW1 | 53 ml |
RNase Free Water | 30 ml |
Skladištenje i stabilnost
This kit is shipped and stored at room temperature and is valid for 12 mjeseca.
Experiment Data
Recenzije
Još nema recenzija.