Uvod
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | IVD5412 precast kit for kingfisher Flex |
| Prijave | RT-PCR，PCR，NGS |
| Proizvodi | Viral DNA / RNA, body cell DNA / RNA |
| Metoda pročišćavanja | Polydisperse magnetic beads |
| Tehnologija pročišćavanja | Magnetic beads technolog |
| Procesna metoda | Ručno ili automatski |
| Vrsta uzorka | |
| Količina uzorka | 200μl |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
Načelo
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.
Prednosti
Sadržaj kompleta
| Cat.No | Reagent | IVD5412-F-96 |
| PK/Carrier RNA | 1x 24 mg/Bottle | |
| Protease Dissolve Buffer Blue | 1.8 ml/Bottle | |
| Tip | 1 | |
| Sample Plate (DW Plate) | 500µl Buffer MLB/Well | 1 |
| Wash 1 Plate (DW Plate) | 500µl Buffer MW1/Well | 1 |
| Wash 2 Plate (DW Plate) | 500µl Buffer CW/Well | 1 |
| Beads Plate (DW Plate) | 500µl CW & 20µl MPN/Well | 1 |
| Elute Plate (KF Plate) | 90µl NFW/Well | 1 |
Skladištenje i stabilnost
This kit is shipped and stored at room temperature and is valid for 12 mjeseca.
Recenzije
Još nema recenzija.