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SPECIFIKACIJA
10 Test/kutija
Kat.br.:MG-CA-R033
NAMJENA
The Feline FPV/FHV/FCV Ab test is a quantitative fluorescence immunoassay used with an Immunofluorescence Analyzer. It determines the concentration of antibodies to feline panleukopenia virus (FPV), feline herpesvirus (FHV), and feline calicivirus (FCV) in serum or plasma samples.
Samo za in vitro dijagnostičku upotrebu. Samo za profesionalnu upotrebu.
PRINCIP ISPITIVANJA
- Ovaj test koristi kvantitativnu tehniku dvostrukih antigenskih sendvič fluorescentnih imunoanaliza.
- The fluorescent signal intensity reflects the amount of FPV-Ab, FHV-Ab, FCV-Ab captured. Koncentracija se izražava kao Tu/ml.
MATERIJAL
| Material Provided | Materijal je potreban, ali nije isporučen |
|---|---|
| 10 individual sealed pouches, each containing: ■ Test Device of FPV-Ab, FHV-Ab, and FCV-Ab (antibody test for Feline Panleukopenia Virus, Feline Herpesvirus, and Feline Calicivirus) ■ Desiccant pouch | 1. Imunofluorescentni analizator (for reading test results) |
| One ID Chip | 2. Odbrojavanje vremena |
| Upute za uporabu | 3. Pipeta (already provided) |
| 10 tubes of FPV/FHV/FCV Ab Sample Buffer | 4. Centrifuga (not typically required for antibody tests) |
| 10 Pipette tips |
ČUVANJE I STABILNOST
- Čuvajte test kit na 4～30 ℃ do datuma isteka.
- Test bi trebao raditi na 18 ~ 28 ℃ nakon otvaranja ispitnog uređaja.
- Nakon što se vrećica otvori, test treba izvesti unutar 30 minuta.
UZIMANJE I PRIPREMA UZORAKA
Test se može izvesti sa serum ili plazma.
- Odvojite serum ili plazmu (Preporučuje se EDTA antikoagulant) od krvi iznutra 3 sati nakon uzimanja krvi. Ako se čini da je uzorak ozbiljno hemoliziran, treba nabaviti i ispitati drugi uzorak.
- Optimalno, test treba provesti odmah nakon uzimanja uzorka. Ako se ispitivanje ne može provesti unutar 3 sati nakon uzimanja krvi, store the specimen at 2～8℃ for no duže od 72 sati. Za dugotrajno skladištenje, uzorci će biti držati ispod -20 ℃.
Stavite sve materijale na sobnu temperaturu prije upotrebe. Smrznuti primjerci moraju biti completely thawed and mixed well prior to testing. Uzorci ne bi trebali biti više puta zamrznuti i odmrznuti. Samo jasno, nehemolizirani uzorci mogu biti koristi se.
POSTUPAK ISPITIVANJA
Preparation:
- Postavite testni uređaj na čisto, vodoravni stol na razini.
- Insert the ID Chip into the meter and click “Read ID chip.” Make sure the Test Device lot number matches the ID Chip number.
Sample Preparation:
- Pipeta 20 μl of prepared sample into FPV/FHV/FCV Ab Sample Buffer.
- Gently mix well. Avoid vigorous agitation and foaming.
Run the Test:
- Choose between Standard Test (a) or Quick Test (b).
(a) Standardni test: * Pipeta 100 μl of mixed sample into the sample well (S) of the FPV-Ab Test Device. * Klik “Standardni test,” insert the FPV-Ab Test Device into the meter holder, i kliknite “Start Test.” Select sample type “Serum/Plasma.” The meter will countdown and read the result automatically. * Repeat for FHV-Ab and FCV-Ab Test Devices, clicking “Započni test” for each after inserting them into the holder.
(b) Brzi test: * Pipeta 100 μl of mixed sample into the sample well (S) of the FPV-Ab Test Device. * Klik “Brzi test,” start the timer immediately. * Leave the FPV-Ab Test Device at room temperature (18°C – 28°C) za 10 minuta. * Insert the FPV-Ab Test Device into the meter holder and click “Start Test.” Select sample type “Serum/Plasma.” Instrument će automatski skenirati uređaj za testiranje i prikazati rezultat testa. * Repeat for FHV-Ab and FCV-Ab Test Devices, inserting them in turn and clicking “Započni test” for each.
- Results:
- Rezultati se prikazuju na glavnom ekranu. Printing is automatic or can be done by clicking “Print.”
KONTROLA KVALITETE
TUMAČENJE
Reference range of FPV/FHV/FCV in feline serum or plasma.
- Raspon detekcije :
① FPV-Ab：4.5 ~640 Tu/ml
② FHV-Ab：5.0 ~640 Tu/ml
③ FCV-Ab：5.0 ~640 Tu/ml
- Referentni raspon:
| Result(Vi/ml) | Level | Prijedlog | ||
| FPV-Ab | FHV-Ab | FCV-Ab | ||
| ≤9 | ≤10 | ≤10 | 0 | No antibody protection vaccination is recommended |
| 9～50 | 10～15 | 10～30 | 1 | The antibody protection is weak, so it is recommended to carry out supplementary immunization or adjust the immunization program |
| 50~ 150 | 15～45 | 30～90 | 2 | |
| 150~ 300 | 45~ 100 | 90~ 200 | 3 | Moderate antibody protection, strengthen antibody level monitoring once every 6 months or so |
| 300~ 500 | 100~ 150 | 200~ 300 | 4 | |
| >500 | >150 | >300 | 5 | The antibody protection is strong, and the antibody level is tested every year to ensure the vaccine protection in time |
Note
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Each laboratory should establish a reference range that represents the populacija koja se procjenjuje.
UPOZORENJA I OGRANIČENJA POSTUPKA
- Ovaj pribor je samo za in vitro dijagnostičku upotrebu.
- Provjerite ambalažu i naljepnice prije uporabe. Nemojte ga koristiti ako je vrećica slomljena, poderan, ili nije dobro zatvoreno, or if the vial looks damaged or leaked.
- Nemojte koristiti uređaj nakon isteka roka valjanosti.
- Jedan vrh pipete treba koristiti samo za jedan uzorak.
- There is the possibility that factors such as technical or procedural errors and additional substances in blood specimens that are not listed in the text may interfere with the test and cause erroneous results.
Recenzije
Još nema recenzija.