- Sample Handling:
- Collect 0.1-1 ml blood sample.
- Priprema uzorka:
- For blood samples:
- Dodati 2-3 times the volume of 1x erythrocyte lysate.
- Fully invert and mix well.
- Centrifuge at 12,000 rpm for 1 minute.
- Carefully remove the supernatant. The precipitate should be white or light red.
- Dodati 400 μl reagent buffer I and 10 μl proteinase K (10 mg/ml) to the precipitate.
- Vortex and mix for 15 sekundi, then incubate at room temperature for 2 minuta.
- For blood samples:
- Special Consideration for Low-Level Biological Blood:
- If processing poultry, bird, or other low-level biological blood with nuclei-containing red blood cells and low sample volume:
- Skip adding 1x erythrocyte lysate.
- Directly add 200 μl of reagent buffer I (total volume not exceeding 500 μl).
- Incubate at room temperature for 2 minuta.
- If processing poultry, bird, or other low-level biological blood with nuclei-containing red blood cells and low sample volume:
- Digestion:
- Dodati 10 μl proteinase K (10 mg/ml) to the mixture.
- Mix thoroughly by inversion.
- Digest at 65°C for 10 minuta, inverting and mixing 6-7 times during digestion until complete.
- Transfer to Reagent Plate:
- Add the lysate from the previous step to the 96-well reagent plate.
- Follow specific steps outlined in the automatic extraction protocol for further processing.
Pre-processing of blood sample genomic DNA extraction 96 Samples
$225.00
Dostava USD 45 - Besplatno preko USD 300DTE je kineska platforma za e-trgovinu specijalizirana za online prodaju molekularnog testiranja, ELISA, i srodni proizvodi.
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| Težina | 0.7 kg |
|---|---|
| naziv marke |