- Sample Handling:
- A. Material Collection & Skladištenje:
- If collected fresh materials cannot be used immediately, cool them in liquid nitrogen and store them at -80°C. Dried materials can be stored in the refrigerator.
- B. Collection Preference:
- Collect fresh material whenever possible, as it contains fewer polysaccharides and polyphenols.
- C. Fungal Sample Collection:
- Fungi can be collected by centrifugation from liquid media.
- A. Material Collection & Skladištenje:
- Priprema uzorka:
- Grind about 100 mg of fresh samples or no more than 20 mg of dry matter with liquid nitrogen.
- Dodati 400 μl Buffer I and 10 μl RNaseA (10 mg/ml) to ensure no lumpy tissue in the sample, which can reduce DNA yield.
- Incubate at 65°C for 2-10 minuta, invert, and mix 2-3 times during this period. Extend incubation time if the sample is difficult to lyse, but not more than 1 sat.
- Dodati 130 μl Buffer II, mix well, and incubate on ice for 5 minutes to precipitate polysaccharides and proteins.
- Purification:
- Add the lysate to the purification column and centrifuge at 13,000 rpm for 2 minuta.
- Transfer to Reagent Plate:
- Add the centrifuged supernatant from the previous step to the 96-well plate.
- Follow specific steps outlined in the automatic extraction protocol for further processing.
Pre-processing of plant sample genomic DNA extraction 96 Samples
$225.00
Dostava USD 45 - Besplatno preko USD 300DTE je kineska platforma za e-trgovinu specijalizirana za online prodaju molekularnog testiranja, ELISA, i srodni proizvodi.
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| Težina | 0.7 kg |
|---|---|
| naziv marke |
Prilog
Povezani proizvodi
- Pročišćavanje DNA i RNA
Pre-processing of Animal tissue/cell tissue sample genomic DNA extraction 96 Samples
$225.00
