Pre-processing of plant sample genomic DNA extraction 96 Samples
Pre-processing of plant sample genomic DNA extraction 96 Samples
Pre-processing of plant sample genomic DNA extraction 96 Samples
Pre-processing of plant sample genomic DNA extraction 96 Samples

Pre-processing of plant sample genomic DNA extraction 96 Samples

$225.00

Dostava USD 45 - Besplatno preko USD 300

DTE je kineska platforma za e-trgovinu specijalizirana za online prodaju molekularnog testiranja, ELISA, i srodni proizvodi.

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Opis

  1. Sample Handling:
    • A. Material Collection & Skladištenje:
      • If collected fresh materials cannot be used immediately, cool them in liquid nitrogen and store them at -80°C. Dried materials can be stored in the refrigerator.
    • B. Collection Preference:
      • Collect fresh material whenever possible, as it contains fewer polysaccharides and polyphenols.
    • C. Fungal Sample Collection:
      • Fungi can be collected by centrifugation from liquid media.
  2. Priprema uzorka:
    • Grind about 100 mg of fresh samples or no more than 20 mg of dry matter with liquid nitrogen.
    • Dodati 400 μl Buffer I and 10 μl RNaseA (10 mg/ml) to ensure no lumpy tissue in the sample, which can reduce DNA yield.
    • Incubate at 65°C for 2-10 minuta, invert, and mix 2-3 times during this period. Extend incubation time if the sample is difficult to lyse, but not more than 1 sat.
    • Dodati 130 μl Buffer II, mix well, and incubate on ice for 5 minutes to precipitate polysaccharides and proteins.
  3. Purification:
    • Add the lysate to the purification column and centrifuge at 13,000 rpm for 2 minuta.
  4. Transfer to Reagent Plate:
    • Add the centrifuged supernatant from the previous step to the 96-well plate.
    • Follow specific steps outlined in the automatic extraction protocol for further processing.

Dodatne informacije

Težina0.7 kg
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