SENO Taq DNA Polymerase PCR Kit 200T

Specification

Taq DNA Polymerase 1000 U with mixed buffer (Shipping with icebag)

Skladištenje Conditions:

Taq DNA Polymerase and Taq PCR Kits should be stored at -30 to -15°C in a constant-temperature freezer upon receipt.

Test:

Notes Before Starting:

• Provided with Load PCR Buffer containing gel-loading reagent and gel-tracking dyes.
• PCR Buffer and Load PCR Buffer yield a final concentration of 1.5 mM MgCl2 in the reaction mix. Adjust Mg2+ concentration if necessary by adding 25 mM MgCl2.
• High-quality PCR-grade dNTP Mix (10 mM) is available separately if needed.
• Keep PCR tubes on ice until placed in the thermal cycler.
• Include a No Template Control (NTC) in every assay.

Preparation and Mixing:

• Thaw buffers and reagents at room temperature or on ice, then keep them on ice after complete thawing.
• Prepare a reaction mix containing all PCR components except template DNA. Prepare 10% more volume than required.
• Mix the reaction mix thoroughly and dispense it into PCR tubes.
• Add template DNA (≤1 µg/reaction) to PCR tubes. For RT-PCR, add an aliquot from the reverse transcriptase reaction, not exceeding 10% of the final PCR volume.

Reaction setup using SENO Taq DNA Polymerase

Thermal Cycling:

Program thermal cycler according to manufacturer’s instructions. Refer to the provided cycling program.

Optimized cycling conditions for Taq DNA Polymerase

Simplified Hot Start:

Start PCR program. Once the thermal cycler reaches 94°C, place PCR tubes in the cycler for improved specificity.

Post-PCR:

• After amplification, samples can be stored at 2–8°C overnight or at -20°C for longer storage.
• PCR products can be directly loaded onto agarose gel using Load PCR Buffer without prior addition of loading buffer and gel-tracking dyes. Refer to the provided table for migration distance and gel tracking dyes.

Migration distance of gel tracking dyes in Load PCR Buffer