Perkenalan
HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, dll.. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, dll..
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation total RNA from bacteria culture |
| Aplikasi | RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation |
| Metode pemurnian | Mini spin column |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Bacteria culture |
| Jumlah sampel | Bacteria: <10^9 |
| Volume elusi | ≥30μl |
| Waktu per lari | ≤40 minutes |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 100mg |
Prinsip
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (seperti guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Akhirnya, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Keuntungan
- Cepat – beberapa sampel dapat diekstraksi 30 menit
- Kemurnian tinggi – the purified RNA can be directly used in various downstream applications
- Pemulihan tinggi – RNA can be recovered at the level of PG
- Pengulangan yang baik – silica gel column purification technology can obtain ideal results every time
- Broad spectrum – it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
- Sufficient components – the kit contains carried lysozyme and glass beads
Isi Paket
| Isi | R418102 | R418103 |
| Waktu Pemurnian | 50 Persiapan | 250 Persiapan |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Tabung Koleksi | 50 | 250 |
| Glass Beads (0.1-0.2mm) | 20 G | 90 G |
| Lysozyme | 90 mg | 400 mg |
| Buffer Larut Protease | 5 ml | 15 ml |
| Buffer STE | 10 ml | 30 ml |
| MagZol Reagent | 60 ml | 270 ml |
| Buffer RW2* | 20 ml | 2 X 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Penyimpanan dan Stabilitas
MagZol Reagent and Lysozyme should be stored at 2-8°C upon arrival. Komponen kit lainnya dapat disimpan kering pada suhu kamar (15-25°C) dan setidaknya stabil 18 bulan dalam kondisi ini. The entire kit can be stored at 2-8°C, tetapi dalam hal ini buffer harus dilarutkan kembali sebelum digunakan. Pastikan semua buffer berada pada suhu ruangan saat digunakan.
Ulasan
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