Perkenalan
HiPure Circulating DNA Midi Kit, designed to efficiently purify short fragments of cell-free DNA (cfDNA) from human biofluids for clinical use:
- Target:
- Tumor-derived cfDNA fragments and mRNAs in blood
- Fetal nucleic acids in maternal blood
- Sample Types:
- Human plasma
- Human serum
- Human urine (fresh or frozen once)
- Focus on Short DNA: Captures short nucleic acids (<1000bp) commonly found in biofluids.
- Efficient Purification: Effectively recovers cfDNA from various biofluids.
- Clinical Applications: Purified cfDNA is suitable for approved in vitro diagnostic (IVD) tests.
Benefits:
- Optimized for Short cfDNA: Ideal for capturing short DNA fragments relevant for cancer biomarkers and liquid biopsies.
- Broad Sample Compatibility: Works with various human biofluids, offering flexibility in sample selection.
- Larger Scale Purification: The Midi Kit format allows the processing of larger sample volumes compared to Mini Kits, potentially increasing cfDNA yield.
- Clinical Utility: Purified DNA can be directly used in approved clinical IVD tests.
This kit empowers researchers and clinical laboratories studying circulating DNA for applications in oncology, non-invasive prenatal testing (NIPT), and liquid biopsies, ultimately aiding in patient diagnosis and management.
Detail
Spesifikasi
Fitur | Spesifikasi |
Fungsi utama | Isolation circulating DNA from 1-5ml plasma, serum, body fluids by spin |
Aplikasi | qPCR, liquid or solid chip analysis, hybridization, and SNP detection, dll.. |
Metode pemurnian | Mini spin column |
Teknologi pemurnian | Teknologi silika |
Metode proses | petunjuk (centrifugation) |
Jenis sampel | Serum, plasma, and other cell-free fluid samples |
Jumlah sampel | 1-5ml |
Volume elusi | ≥50μl |
Waktu per lari | ≤70 minutes |
Volume pembawa cairan per kolom | 4ml |
Hasil pengikatan kolom | 1mg |
Prinsip
This product utilizes silica gel columns for efficient DNA extraction:
- Lysis & Digestion: The sample undergoes treatment with a special solution and enzyme to break open cells and degrade proteins, releasing DNA.
- Binding to Silica: The mixture is transferred to a column. The silica membrane in the column captures DNA molecules, while other substances pass through.
- Washing: Wash buffers to remove leftover proteins and cellular debris.
- Elution: A low-salt buffer elutes the purified DNA from the silica, collecting it in a separate tube.
This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis.
Keuntungan
- Hasil tinggi – most optimal process, free DNA (>50bp) can be obtained to the maximum extent
- Konsentrasi tinggi – low elution volume, ensuring high nucleic acid concentration
- Kemurnian tinggi – low alcohol binding method, completely removing inhibitor and protein pollution
- Pemulihan tinggi – DNA can be recovered at the level of PG by silica gel column purification
Isi Paket
Isi | D318203D |
Waktu Pemurnian | 50 Persiapan |
Buffer ACL | 250 ml |
Buffer ACB* | 300 ml |
Buffer DCW1* | 22 ml |
Buffer DCW2* | 10 ml |
Proteinase K | 540 mg |
Buffer Larut Protease | 30 ml |
Carrier RNA | 110 μg |
Nuclease Free Water | 20 ml |
HiPure CFDNA Mini Columns | 50 |
2 ml Tabung Koleksi | 100 |
Extender Tubes | 50 |
Sealing O-Ringes | 50 |
50ml Collection Tube | 50 |
Support Tube | 50 |
Penyimpanan dan Stabilitas
- Proteinase K & Carrier RNA: Fridge (2-8°C) for best results.
- Everything Else: Suhu kamar (15-25°C) is fine.
- Important: If storing cold, warm buffers before use.
Ulasan
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