HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, pengurutan,restriction digestion, or various labeling reactions. In addition, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Recover DNA fragments between 20bp-20kbp from crude DNA and enzymatic reaction solution |
| Aplikasi | PCR, pengurutan, labeling reactions, ligations and restriction digestion, dll.. |
| Metode pemurnian | Mini spin column |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Crude DNA, enzymatic reaction solution |
| Jumlah sampel | <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”><150μl |
| Recovery | 80% |
| Volume elusi | <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≥15μl |
| Waktu per lari | <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≤10 minutes |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 20mg |
Prinsip
The HiPure system uses a simple bind-wash-elute procedure. Gelslices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Keuntungan
- High recovery efficiency – hingga 80% DNA recovery
- Cepat – isolation can be completed in 10 minutes by column gel method
- Low elution volume – elution volume can be as low as 10μl
- Efficient – efficient removal of various inhibitory factors
Isi Paket
| Isi | D214102 | D214103 |
| Waktu Pemurnian | 100 Persiapan | 250 Persiapan |
| Buffer GWP | 30 ml | 60 ml |
| Buffer DW2 | 20 ml | 50 ml |
| Penyangga Elusi | 30 ml | 60 ml |
| HiPure DNA Mini Columns I | 50 | 250 |
| 2 ml Tabung Koleksi | 50 | 250 |
Penyimpanan dan Stabilitas
HiPure DNA Clean Up Kit components are guaranteed for at least one year when stored at room temperature. Jika ada endapan yang terbentuk di buffer, hangatkan pada suhu 37℃ hingga larut.
Ulasan
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