Ikhtisar Produk
- Offers fast and easy methods for purifying total DNA.
- Ensures reliable PCR and Southern blotting results.
- Capable of purifying various types of DNA, including genomic, viral, and mitochondrial.
- Can purify DNA from small volumes of blood, jaringan, and dry blood spots.
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation total DNA from 1-10μl blood, <10mg tissue, urine, blood stain, seminal stain |
| Aplikasi | PCR, southern bolt and virus detection, dll.. |
| Metode pemurnian | Mini spin column |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Jaringan hewan, blood stain, urine, seminal stain and various forensic samples |
| Jumlah sampel | Darah:1-100μl, Jaringan:<10mg |
| Volume elusi | |
| Waktu per lari | |
| Volume pembawa cairan per kolom | |
| Hasil pengikatan kolom |
Prinsip
- Utilizes silica column purification method.
- Sample is lysed and digested with lysate and protease, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- Nucleic acid is adsorbed on the membrane, sedangkan protein tidak diserap dan dihilangkan dengan filtrasi.
- Proteins and other impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10mm Tris, pH 9.0, 0.5mm EDTA).
Keuntungan
- Cepat – beberapa sampel dapat diekstraksi 20 menit (after digestion)
- Kemurnian tinggi – purified DNA can be directly used in various downstream applications
- Pemulihan tinggi – DNA dapat dipulihkan pada tingkat PG
- Pengulangan yang baik – teknologi silika dapat memperoleh hasil yang ideal setiap saat
Isi Paket
| Isi | D312502 | D312503 |
| Waktu Pemurnian | 50 Persiapan | 250 Persiapan |
| Buffer ATL | 15 ml | 60 ml |
| Buffer AL | 15 ml | 60 ml |
| Buffer GW1* | 22 ml | 66 ml |
| Penyangga GW2* | 20 ml | 2 X 50 ml |
| Carrier RNA | 310 μg | 2 X 310 mg |
| Proteinase K | 24 mg | 120 mg |
| Buffer Larut Protease | 1.8 ml | 10 ml |
| Penyangga AE | 15 ml | 60 ml |
| HiPure DNA Mini Columns I | 50 | 2 X 125 |
| 2 ml Tabung Koleksi | 100 | 5 X 100 |
Penyimpanan dan Stabilitas
- Carrier RNA and Proteinase K should be stored at 2–8°C upon arrival.
- Short-term storage at room temperature (15–25°C) for up to 12 weeks does not affect their performance.
- Remaining kit components can be stored dry at room temperature (15–25°C) and remain stable for at least 18 bulan dalam kondisi ini.
Data Eksperimen
Ulasan
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