Perkenalan
HiPure FFPE RNA Kit supplies a simple and rapid Ekstraksi RNA for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 menit (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation total RNA from FFPE tissue |
| Aplikasi | RT-PCR, cDNA synthesis, second generation sequencing |
| Produk | RNA, miRNA |
| Metode pemurnian | Mini spin column |
| Teknologi pemurnian | Teknologi silika, DNase |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | FFPE slice, FFPE embedded tissue |
| Jumlah sampel | Jaringan: <6 mg FFPE: <6 slices |
| Menghasilkan | 1-20μg |
| Volume elusi | ≥15μl |
| Waktu per lari | ≤30 menit (after digestion) |
| Volume pembawa cairan per kolom | 800μl |
Prinsip
This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 menit. Setelah lisis, samples are incubated at 80ºC for 15 menit. Transfer to an adsorption column and RNA is adsorbed on the membrane, sedangkan protein tidak diserap dan dihilangkan dengan filtrasi. Setelah mencuci protein dan kotoran lainnya, RNA wasfinally eluted with low-salt buffer.
Keuntungan
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- High quality – high purity total RNA can be directly used in various sensitive downstream applications
- Cepat – beberapa sampel dapat diekstraksi 60 minutes by column method
- Aman – no phenol chloroform extraction required
- Sensitive – RNA can be recovered at the level of PG
Isi Paket
| Isi | IVD4144 |
| Waktu Pemurnian | 50 Persiapan |
| HiPure RNA Micro Columns | 50 |
| 2ml Tabung Koleksi | 50 |
| Proteinase K | 24 mg |
| Buffer Larut Protease | 1.8 ml |
| DNase I | 600 μl |
| DNase Booster Buffer | 1.5 ml |
| Buffer DPS | 60 ml |
| Buffer FRL | 15 ml |
| Buffer RLC | 15 ml |
| Buffer RWC* | 10 ml |
| Buffer RW2* | 20 ml |
| RNase Free Water | 10 ml |
Penyimpanan dan Stabilitas
Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. Namun, penyimpanan jangka pendek (DNase I up to 1 minggu, Proteinase K up to 8 minggu) pada suhu kamar(15–25°C) tidak mempengaruhi kinerja mereka. The remaining kit components can be stored at roomtemperature (15–25°C) dan setidaknya stabil 18 bulan dalam kondisi ini.
Data Eksperimen
Ulasan
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