The HiPure Fruit RNA Mini Kit provides fast purification of high-quality RNA from fruits, cell, tisu, and yeast using silica-membrane spin columns with a binding capacity of 100µg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA(mRNA) purification, nuclease protection and in vitro translation.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation total RNA from <150 mg fruit or seed using chloroform and Plantzol reagent |
| Aplikasi | RT-PCR, qPCR, Northern hybridization, Poly A purification, nucleic acid protection and in vitro translation translation |
| Metode pemurnian | Mini spin column |
| Teknologi pemurnian | Teknologi silika, DNA filtration technology |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Simple plant, petal, peel, seed |
| Jumlah sampel | 50-150mg |
| Volume elusi | ≥300μl |
| Waktu per lari | ≤15 minutes（1-24 samples） |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 100mg |
Prinsip
The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 15 menit. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 300µl water using the Kit.
Keuntungan
- Efficient DNA removal – one step Ekstraksi RNA can effectively remove genomic DNA
- High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Cepat – beberapa sampel dapat diekstraksi 15 minutes by column method
- Wide range – samples including animals, plants, bacteria, cells, dll.
- High output – large sample amount, and the yield can be up to 200μg
Isi Paket
| Isi | R401402 | D401403 |
| Waktu Pemurnian | 50 Persiapan | 250 Persiapan |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Tabung Koleksi | 50 | 250 |
| PlantZol Reagent | 60 ml | 200 ml |
| Buffer GXP | 30 ml | 150 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2* | 20 ml | 2 X 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Penyimpanan dan Stabilitas
The Kit can be stored dry at room temperature (15–25°C) dan setidaknya stabil 18 bulan dalam kondisi ini. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
Ulasan
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