HiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 menit. Purified DNA can be used directly for PCR, RSK, AFLP, RAPD and Southern Blot, dll..
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation total DNA from 150 mg plant and fungal tissue |
| Aplikasi | PCR, RSK, AFLP, RAPD dan noda selatan, dll.. |
| Metode pemurnian | Mini spin column |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Segar / sampel tanaman beku, dried plant / sampel benih |
| Jumlah sampel | Segar / sampel tanaman beku: 50-150 mg Dried plant / sampel benih: 15-40 mg |
| Volume elusi | ≥40μl |
| Waktu per lari | 30-50 menit |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 100μg |
Prinsip
This product is based on silica column purification. The sample is lysed with CATB Buffer. DNA is released into the lysate. Cell debris, precipitated proteins and polysaccharides are removed by chloroform extraction. After adjust the binding condition, transfer to an adsorption column. DNA is adsorbed on the membrane, sedangkan protein tidak diserap dan dihilangkan dengan filtrasi. Setelah mencuci protein dan kotoran lainnya, asam nukleat akhirnya dielusi dengan buffer rendah garam (10mm Tris, pH9.0, 0.5mm EDTA).
Keuntungan
- Broad spectrum – suitable for extracting DNA from various plant samples
- Cepat – beberapa sampel dapat diekstraksi 30 menit dengan teknologi silika
- Pengulangan yang baik – silica gel column purification technology can obtain ideal results every time
Isi Paket
| Isi | D318702 | D318703 |
| Waktu Pemurnian | 50 Persiapan | 250 Persiapan |
| Buffer PAL | 60 ml | 200 ml |
| Buffer GWP | 60 ml | 200 ml |
| Buffer DW1 | 30 ml | 150 ml |
| Penyangga GW2* | 20 ml | 50 ml |
| Penyangga AE | 20 ml | 60 ml |
| Kolom Mini DNA HiPure II | 50 | 250 |
| 2 ml Tabung Koleksi | 50 | 250 |
Penyimpanan dan Stabilitas
This product can be stored dry at room temperature (15-25°C) dan setidaknya stabil 18 bulan dalam kondisi ini.
Panduan Pembelian
| Nama | TIDAK KUCING | Segar / jumlah jaringan beku | Jumlah tisu kering | Jenis kolom | Volume elusi | Menghasilkan (otot) |
| HiPure HP Plant DNA Kit | D3187 | 150mg | 40mg | 1.5kolom ml | 50 – 100μl | 3-70μg |
| Kit Maxi DNA Tanaman HP HiPure | D3163 | 5G | 1G | 50kolom ml | 0.7 – 3ml | 75-1250μg |
| Kit DNA Tanaman HiPure SF | D3164 | 100mg | 20mg | 1.5kolom ml | 50 – 100μl | 3-50μg |
| DNA Tanaman HiPure SF 96 Perlengkapan | D3167 | 50mg | 15mg | 96 piring sumur | 75 – 150μl | 3-30μg |
Ulasan
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