HiPure HP Plant RNA Mini Kit combines guanidine isothiocyanate lysing and silica gel membrane purification technology to simplify total Ekstraksi RNA. Ultracentrifugation in CsCl purification and LiCl / ethanol precipitation are not required. The kit uses DNase digestion to completely remove DNA. It is suitable for extracting up to 100μg of total RNA (including miRNA) from plant samples less than 200mg. Several samples can be extracted within 40 menit. The purified RNA can be directly used for RT-PCR, fluorescent quantitative RT-PCR, Northern hybridization, second generation sequencing, dll..
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation total RNA (include miRNA) from <200mg difficult-to-extract plant samples (use low toxicity chloroform substitutes) |
| Aplikasi | RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation |
| Metode pemurnian | Mini spin column |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Hard-to-extraction plant samples such as fruit and seed, grape leaves, tea |
| Jumlah sampel | ≤200 mg |
| Volume elusi | ≥30μl |
| Waktu per lari | 1-24 samples within 30 menit |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 100mg |
Prinsip
This kit uses glass fiber filter membrane purification technique, and only requires simple combination-washing-elution steps. The sample is lysed and homogenized in the solution containing guanidine salt, ethanol is added to provide appropriate binding conditions, and transferred to the purification column for centrifugation. Up to 100µg of RNA can be selectively bound to the membrane, pollutants are efficiently washed off after three times of washing, and finally the purified RNA is eluted by RNase Free Water.
Keuntungan
- Completely remove DNA by using of DNase
- High quality – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Cepat – beberapa sampel dapat diekstraksi 40 minutes by column method
- Sensitive – RNA can be recovered at the level of PG
- Broad spectrum – various types of plant samples can be processed by diversity of operating procedures
Isi Paket
| Isi | R416502 | D416503 |
| Waktu Pemurnian | 50 Persiapan | 250 Persiapan |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Tabung Koleksi | 100 | 250 |
| DNase I | 600 μl | 5 X 600 μl |
| DNase Buffer | 6 ml | 30 ml |
| Buffer PAL | 60 ml | 270 ml |
| Buffer GXP2* | 20 ml | 100 ml |
| Buffer BDP | 60 ml | 270 ml |
| Buffer RW1 | 50 ml | 250 ml |
| Buffer RW2* | 20 ml | 2 X 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Penyimpanan dan Stabilitas
DNase I should be stored at -20-8°C upon arrival. The remaining kit components can be stored at room temperature (15-25°C) dan setidaknya stabil 18 bulan dalam kondisi ini. The entire kit can be stored at 2-8°C, tetapi dalam hal ini buffer harus dilarutkan kembali sebelum digunakan. Pastikan semua buffer berada pada suhu ruangan saat digunakan.
Ulasan
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