Perkenalan
The HiPure Liquid RNA & miRNA Kit integrates phenol/guanidine-based sample lysis and silica-membrane purification of total RNA. MagZol LS Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate designed to facilitate lysis of liquid sample and inhibit RNase. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable the use of up to 0.25ml liquid sample per mini spin column.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation of total RNA from 0.25ml body fluids using column and MagZol LS reagent |
| Aplikasi | qPCR / RT-PCR, liquid or solid-phase chip analysis, hybridization, and SNP detection |
| Metode pemurnian | Kolom putaran mini |
| Teknologi pemurnian | Teknologi silika, acid phenol / guanidine extraction technology (MagZol pretreatment technology) |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Darah, serum, plasma |
| Jumlah sampel | 250μl |
| Volume elusi | ≥20μl |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 100μg |
Prinsip
0.25ml liquid samples are homogenized in 0.75ml MagZol LS Reagent. After the addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (hingga 100 mg) binds to the membrane, and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30–100µl of RNase-free water.
Keuntungan
- High quality – high purity total RNA can be directly used in various sensitive downstream applications
- Fast – isolation of several samples can be completed in 40 minutes by using the column purification method
- Keamanan – no phenol-chloroform extraction is required
- Sensitive – direct lysis of blood, plasma, and other samples without separation of leukocytes
Isi Paket
| Isi | R416302 | R416303 |
| Waktu Pemurnian | 50 Persiapan | 250 Persiapan |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Tabung Koleksi | 50 | 2 X 125 |
| MagZol LS Reagent | 60 ml | 270 ml |
| Buffer RWC | 20 ml | 60 ml |
| Buffer RW2* | 20 ml | 2 X 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Penyimpanan dan Stabilitas
MagZol LS Reagent should be stored at 2-8°C upon arrival. Namun, penyimpanan jangka pendek (hingga 24 minggu) pada suhu kamar (15-25°C) tidak mempengaruhi kinerja mereka. Komponen kit lainnya dapat disimpan kering pada suhu kamar (15-25°C) dan setidaknya stabil 18 bulan dalam kondisi ini.
Ulasan
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