Perkenalan
Hipure Microbial RNA kit is suitable for extracting high-purity total RNA from bacterial/yeast culture medium. This kit combines high-efficiency Magzol Reagent (one-step extraction reagent) and silicagel column purification technology to complete the extraction of high-purity total RNA in only 40 menit. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation total RNA from bacteria, yeast cells |
| Aplikasi | RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation |
| Metode pemurnian | Mini spin column |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Bacteria, yeast cells |
| Jumlah sampel | Bacteria: <10^9；Yeast cells：<2 x10^7 |
| Volume elusi | ≥30μl |
| Waktu per lari | ≤40 minutes |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 100mg |
Prinsip
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of a high concentration of ionizing agent (seperti guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Akhirnya, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Keuntungan
- Cepat – beberapa sampel dapat diekstraksi 30 menit
- Kemurnian tinggi – the purified RNA can be directly used in various downstream applications
- Pemulihan tinggi – RNA can be recovered at the level of PG
- Pengulangan yang baik – silica gel column purification technology can obtain ideal results every time
- Broad spectrum – it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
- Sufficient components – the kit contains carried wall-breaking enzymes and glass beads
Isi Paket
| Isi | R418202 | R418203 |
| Waktu Pemurnian | 50 Persiapan | 250 Persiapan |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Tabung Koleksi | 50 | 250 |
| Glass Beads (0.1-0.6mm) | 30 G | 150 G |
| DNase I | 600 μl | 5 X 600 μl |
| DNase Buffer | 6 ml | 30 ml |
| Buffer Larut Protease | 1.8 ml | 15 ml |
| Buffer ATL | 50 ml | 200 ml |
| Buffer PHC | 50 ml | 200 ml |
| Buffer GXP2* | 20 ml | 100 ml |
| Buffer RW1 | 50 ml | 250 ml |
| Buffer RW2* | 20 ml | 2 X 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Penyimpanan dan Stabilitas
Buffer PHC should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. Namun, penyimpanan jangka pendek (DNase I up to 1 minggu, Buffer PHC up to 12 minggu) pada suhu kamar(15–25°C) tidak mempengaruhi kinerja mereka. The remaining kit components can be stored at roomtemperature (15–25°C) dan setidaknya stabil 18 bulan dalam kondisi ini.
Ulasan
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