Perkenalan
- HiPure DNA plasmid Plus 96 Perlengkapan:
- Enables up to 96 mini-preps to be performed simultaneously.
- Process completed in less than 45 minutes using the Vacuum Manifold (Qiavac 96).
- Provides a fast, sederhana, and cost-effective method for high-throughput plasmid DNA extraction in routine molecular biology laboratory applications.
- Key Features:
- Utilizes silica-membrane technology, eliminating cumbersome steps associated with loose resins or slurries.
- Plasmid DNA purified with the kit is immediately ready for use.
- Eliminates the need for phenol extraction and ethanol precipitation.
- High-quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation up to 20µg plasmid DNA from 1.5ml bacterial culture using 96 well bind plate and 96 filterplate |
| Aplikasi | Pencernaan enzim, pengurutan, PCR, labeling, dll.. |
| Metode pemurnian | 96 piring sumur |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Plasmid konvensional, plasmid kurang dari 30KB |
| Jumlah sampel | 1-1.5ml（x96） |
| Menghasilkan | 1-15µg/1ml |
| Volume elusi | ≥70μl |
| Waktu per lari | ≤60 menit |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 70mg |
Prinsip
- HiPure Plasmid Procedure:
- Based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt.
- Utilizes a unique silica membrane that replaces glass or silica slurries for plasmid DNA minipreps.
- Procedure Steps:
- Preparation and Clearing of Bacterial Lysate:
- The alkaline method is used to prepare and clear bacterial lysate.
- DNA Binding:
- Supernatant transferred to a column to bind DNA to the silica membrane.
- Washing and Elution:
- Proteins and other impurities washed away.
- Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
- Preparation and Clearing of Bacterial Lysate:
Keuntungan
- Kemurnian tinggi – Plasmid yang dimurnikan dapat langsung digunakan dalam pengurutan, PCR pencernaan enzim, dll..
- Cepat – hanya dibutuhkan 60 minutes to complete the isolation
- Hasil tinggi – up to 1mg plasmid can be bound in one column
- Economy – high cost-performance
Isi Paket
| Isi | P100601 | P100602 | P100603 |
| Waktu Pemurnian | 1 X 96 Persiapan | 4 X 96 Persiapan | 20 X 96 Persiapan |
| RNase A | 5 mg | 20 mg | 100 mg |
| Penyangga P1 | 30 ml | 120 ml | 600 ml |
| Penyangga P2 | 30 ml | 120 ml | 600 ml |
| Penyangga P3 | 40 ml | 180 ml | 800 ml |
| Penyangga PW1 | 100 ml | 500 ml | 2 X 1000 ml |
| Buffer PW2 | 50 ml | 2 X 100 ml | 4 X 200 ml |
| Penyangga Elusi | 150 ml | 60 ml | 300 ml |
| Lysate Clear Plate | 1 | 4 | 20 |
| Pelat DNA HiPure | 1 | 4 | 20 |
| 1.6 ml Collection Plate | 1 | 4 | 20 |
| 0.5ml Elute Plate | 1 | 4 | 20 |
Penyimpanan dan Stabilitas
Komponen kit dapat disimpan kering pada suhu kamar (15-25°C) dan setidaknya stabil 18 bulan dalam kondisi ini. Jika ada endapan yang terbentuk di buffer, hangatkan pada suhu 37℃ hingga larut. Setelah penambahan RNase A， Buffer P1 stabil untuk 6 bulan bila disimpan pada suhu 2-8°C.
Panduan Pembelian
| Nama | TIDAK KUCING | Jumlah sampel | Jenis kolom | Volume elusi | Menghasilkan | Waktu per lari | Persyaratan sentrifugasi |
| Kit Mini Plasmid HiPure | P1001 | 1-5ml | 1.5kolom ml | 30-50μl | 5–35µg/5ml | ≤30 menit | mesin sentrifugal kecil |
| Kit Mini Plasmid HiPure II | P1002 | 5-15ml | 1.5kolom ml | 75-100μl | 20-80mg/15ml | ≤30 menit | mesin sentrifugal kecil |
| HiPure Plasmid Plus 96 Perlengkapan | P1006 | 1-1.5ml | 96 piring sumur | 70-150μl | 1-15μg/1ml | ≤60 menit | 96-centrifuge pelat sumur |
| Kit Midi Plasmid Fastfilter HiPure | P1013 | 30-50ml | 15kolom ml | 0.3-0.8ml | 50–250µg/50ml | ≤60 menit | 15ml sentrifugasi |
| Kit Maxi Plasmid Fastfilter HiPure | P1014 | 100-200ml | 50kolom ml | 0.5-1.5ml | 0.4–1mg/200ml | ≤60 menit | 50ml sentrifugasi |
Ulasan
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