Perkenalan
HiPure RNA Clean Up Kit provides a rapid and easy method for the purification and concentrate RNA from enzymatic reactions or for desalting the RNA samples. RNA purified using HiPure RNA Clean Up Kit is ready for all downstream applications such as RT-PCR, Northern blotting, mRNA purification, nuclease protection, and in vitro translation.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Recovery of single stranded or double stranded RNA from crude RNA products and enzymatic reaction solution |
| Aplikasi | Pengurutan, ligasi, enzyme digestion, RT-PCR, labeling, dll.. |
| Metode pemurnian | Mini spin column |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Crude RNA, enzymatic reaction solution |
| Jumlah sampel | ≤100μl |
| Recovery | 90% |
| Volume elusi | ≥15μl |
| Waktu per lari | ≤15 minutes |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 100μg |
Prinsip
The HiPure system uses a simple bind-wash-elute procedure. Binding buffer is added directly to the sample or other enzymatic reaction, and the mixture is applied to the column. Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure RNA is eluted with a small volume of low-salt buffer provided or water, ready to use in all subsequent applications.
Keuntungan
- Pemulihan tinggi – recovery of RNA up to 90%
- Low elution volume – the elution volume can be as low as 15µl
- Cepat – only 10 minutes for recovery by using column method
Isi Paket
| Isi | R214402 | R214403 |
| Waktu Pemurnian | 50 Persiapan | 250 Persiapan |
| Buffer GXP | 30 ml | 120 ml |
| Buffer RW2 | 20 ml | 2 X 50 ml |
| RNase-Free Water | 20 ml | 60 ml |
| HiPure RNA Mini Columns I | 50 | 250 |
| 2 ml Tabung Koleksi | 50 | 250 |
Penyimpanan dan Stabilitas
The Kit can be stored dry at room temperature (15-25°C) dan setidaknya stabil 18 bulan dalam kondisi ini. The entire kit can be stored at 2-8°C, tetapi dalam hal ini buffer harus dilarutkan kembali sebelum digunakan. Pastikan semua buffer berada pada suhu ruangan saat digunakan.
Ulasan
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