Jaringan HiPure&Blood DNA Maxi Column Kit

• Challenges in DNA Extraction from Blood Samples:
  1. High Infectious Risk: Blood samples pose a significant infectious hazard to operators and the environment.
  2. Complex DNA Source: Blood DNA contains various types such as mitochondrial, genomik, and circulating DNA, leading to potential loss during extraction.
  3. Presence of Impurities: Blood samples contain impurities and inhibitory factors that can interfere with DNA extraction and downstream applications.
• Existing Extraction Methods:
  • Phenol-chloroform extraction, salting out, dll..
  • These methods typically involve pre-treatment steps to remove red blood cells and isolate white blood cells, which may result in loss of nucleic acid information.
• Product Benefits:
  • Fast and Easy Purification: Provides a rapid and straightforward method for total pemurnian DNA.
  • Suitability: Purifies total DNA (genomik, viral, mitokondria) from whole blood, jaringan, and cultured cells.
  • Aplikasi: Ensures reliable DNA for PCR and Southern blotting assays.

Spesifikasi

Prinsip

1. Cell Breakdown: Your sample is lysed (broken open) and digested with enzymes to release DNA into solution.
2. Capture: The mixture is passed through a silica column. DNA sticks to the silica membrane, while proteins and other impurities don’t.
3. Washing Away Impurities: The column is washed to remove any remaining proteins and other unwanted molecules.
4. DNA Release: Pure DNA is eluted (washed off) the silica membrane with a special buffer solution. This purified DNA is then ready for further analysis.

Keuntungan

• High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, dll..
• Cepat – without separation of leukocytes, organic extraction, or ethanol precipitation
• Simple – all nucleic acids can be obtained by direct digestion
• Pertinence – specially designed for isolating DNA from 3-10ml blood and related body fluids
• Penerapan yang luas – handle a variety of liquid samples
• Economy – excellent cost-effectiveness performance

Isi Paket

Penyimpanan dan Stabilitas

Storage Recommendations:

• Proteinase K and RNase A: Upon arrival, store at 2-8°C. Short-term storage (hingga 12 minggu) pada suhu kamar (15-25°C) is acceptable without affecting performance.
• Remaining Kit Components: Can be stored at room temperature (15-25°C) and remain stable for at least 18 bulan dalam kondisi ini.

Panduan Pembelian