This kit provides fast purification of high-quality RNA from cells, tisu, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation total RNA (not include miRNA) from 20mg tissue, 150mg plant, 5 X 106 cell using two columns (gDNA removed column) |
| Aplikasi | RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing |
| Metode pemurnian | Mini spin column |
| Teknologi pemurnian | Teknologi silika, DNA filtration technology |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Animal soft tissue, cultured cells, lymphocytes, simple plant tissue |
| Jumlah sampel | Cells:≤1 x 107 Animal tissue sample: 1-20 mg Plant tissue: 50-150 mg |
| Menghasilkan | 2-100μg |
| Volume elusi | ≥50μl |
| Waktu per lari | ≤25 minutes（1-24 samples） |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 100mg |
Prinsip
The Kit isolates total RNA from up to 107 cells or 20 mg tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 menit. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.
Keuntungan
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- High quality – high purity total RNA can be directly used in various sensitive downstream applications
- Cepat – beberapa sampel dapat diekstraksi 25 minutes by column method
- Aman – no phenol chloroform extraction required
- Sensitive – RNA can be recovered at the level of PG
Isi Paket
| Isi | R411102 | D411103 |
| Waktu Pemurnian | 50 Persiapan | 250 Persiapan |
| HiPure DNA Mini Columns | 50 | 250 |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Tabung Koleksi | 100 | 2 X 250 |
| Buffer RLC | 50 ml | 200 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2* | 12 ml | 2 X 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Penyimpanan dan Stabilitas
HiPureKit can be stored dry at room temperature (15-25°C) and are stable for at least18 months under these conditions. During shipment, crystals or precipitationmay form in the Buffer RLC. Dissolve by warming buffer to 37°C.
Ulasan
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