Perkenalan
This product highlights a versatile DNA extraction product suitable for various sample types:
- Tissues
- Cells
- Darah (including whole blood, plasma, serum, buffy coat)
- Saliva
- Swabs
- Blood Spots
- Semen
- Other clinical samples
Key Features:
- Rapid Extraction: Streamlined protocol ensures quick DNA isolation with minimal hands-on time.
- High-Quality DNA: Purifies total DNA (genomik, viral, mitokondria) for reliable results in downstream applications like:
- PCR (Reaksi Berantai Polimerase)
- Quantitative PCR (qPCR)
- Southern Blotting
- Viral DNA detection
- Other DNA-based analyses
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation of total DNA from tissue/blood/body fluid/swab/dry blood spots |
| Aplikasi | PCR, qPCR, southern bolt and virus detection, dll.. |
| Metode pemurnian | Mini spin column |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Jaringan, cells, darah, saliva, swabs, blood spots, semen, and other clinical samples |
| Jumlah sampel | Solid tissue: 1-10mg, Anticoagulant blood: 200μl |
| Menghasilkan | 1 – 15mg |
| Volume elusi | <span “=””>≥20μl |
| Waktu per lari | 30 – 60 menit |
| Volume pembawa cairan per kolom | 750μl |
| Hasil pengikatan kolom | 100mg |
Prinsip
- Produk ini didasarkan pada pemurnian kolom silika.
- The sample is lysed and digested with lysate and protease, and DNA is released into the lysate.
- Transfer to an adsorption column.
- Nucleic acid is adsorbed on the membrane, sedangkan protein tidak diserap dan dihilangkan dengan filtrasi.
- Setelah mencuci protein dan kotoran lainnya, asam nukleat akhirnya dielusi dengan buffer rendah garam (10mm Tris, pH 9.0, 0.5mm EDTA).
Keuntungan
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, dll..
- Cepat – without separation of leukocytes, organic extraction, or ethanol precipitation
- Simple – all nucleic acids can be obtained by direct digestion
- Penerapan yang luas – It can handle various liquid samples, animal tissues, and cultured cells
Isi Paket
| Isi | IVD3018 |
| Waktu Pemurnian | 100 |
| HiPure DNA Mini Columns I | 100 |
| 2ml Tabung Koleksi | 2 X 100 |
| Buffer ATL | 60 ml |
| Buffer AL | 60 ml |
| Penyangga GW1 | 44 ml |
| Penyangga GW2 | 50 ml |
| Proteinase K | 60 mg |
| Buffer Larut Protease | 5 ml |
| Penyangga AE | 15 ml |
Penyimpanan dan Stabilitas
Proteinase K should be stored at 2–8°C upon arrival. Namun, penyimpanan jangka pendek (hingga 12 minggu) pada suhu kamar (15–25°C) tidak mempengaruhi kinerja mereka. The remaining kit components can be stored at room temperature (15–25°C) dan setidaknya stabil 18 bulan dalam kondisi ini. Seluruh kit dapat disimpan pada suhu 2–8°C, but in this case, buffers should be redissolved before use. Pastikan semua buffer berada pada suhu ruangan saat digunakan.
Data Eksperimen
