Ikhtisar Produk
- Suitable for rapid extraction of total DNA from various sources: jaringan, cells, darah, saliva, swabs, blood spots, semen, and other clinical samples.
- Extracted DNA can be used directly for PCR, quantitative PCR, Southern Blot, virus DNA testing, dan aplikasi lainnya.
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation of total DNA from tissue/blood/body fluid/swab/dry blood spots |
| Aplikasi | PCR, qPCR, southern bolt and virus detection, dll. |
| Metode pemurnian | Mini spin column |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Jaringan, cells, darah, saliva, swabs, blood spots, semen, and other clinical samples |
| Jumlah sampel | Solid tissue: 1-10mg; Anticoagulant blood: 200μl |
| Volume elusi | ≥20μl |
| Waktu per lari | 30 – 60 menit |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 100mg |
Prinsip
- Utilizes silica column purification method.
- The sample is lysed and digested with lysate and protease, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and removed through filtration.
- Proteins and impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10mm Tris, pH 9.0, 0.5mm EDTA).
Keuntungan
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, dll..
- Cepat – without separation of leukocytes, organic extraction, or ethanol precipitation
- Simple – all nucleic acids can be obtained by direct digestion
- Penerapan yang luas- handle a variety of liquid samples
Isi Paket
| Isi | D301802 | D301803 |
| Waktu Pemurnian | 50 | 250 |
| HiPure DNA Mini Columns I | 50 | 250 |
| 2ml Tabung Koleksi | 100 | 500 |
| Buffer ATL | 30 ml | 150 ml |
| Buffer AL | 30 ml | 150 ml |
| Penyangga GW1 | 22 ml | 88 ml |
| Penyangga GW2 | 12 ml | 50 ml |
| Proteinase K | 24 mg | 120 mg |
| Buffer Larut Protease | 1.8 ml | 10 ml |
| Penyangga AE | 15 ml | 60 ml |
Penyimpanan dan Stabilitas
- Proteinase K should initially be stored at 2-8°C upon arrival.
- Short-term storage at room temperature (15-25°C) for up to 12 weeks does not affect its performance.
- The remaining kit components can be stored at room temperature (15-25°C) and remain stable for at least 18 bulan.
- Alternatively, the entire kit can be stored at 2-8°C, but buffers should be redissolved before use.
- Ensure all buffers are at room temperature when used.
Data Eksperimen
Ulasan
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