Perkenalan
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, dll., the extracted products can be used for clinical in vitro detection.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Extract viral RNA/DNA from 200μl plasma/serum samples |
| Aplikasi | RT-PCR，PCR，NGS |
| Produk | Viral DNA / RNA, body cell DNA / RNA |
| Metode pemurnian | Mini spin column |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Plasma, serum, effusion, urine, fecal suspension supernatant |
| Jumlah sampel | 200μl |
| Volume elusi | ≥30μl |
| Waktu per lari | |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 100μg |
Prinsip
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA/RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, sedangkan protein tidak diserap dan dihilangkan dengan filtrasi. Setelah mencuci protein dan kotoran lainnya, DNA / RNA was finally eluted with low-salt buffer (10 MmTris, pH 8.0).
Keuntungan
- Cepat – column purification without PK digestion
- High quality – high purity total RNA can be directly used in various sensitive downstream applications
- Aman – no phenol chloroform extraction required
- Sensitive – DNA/RNA can be recovered at the level of PG
Isi Paket
| Isi | IVD4175 |
| Waktu Pemurnian | 100 Persiapan |
| HiPure Viral Column | 100 |
| 2ml Tabung Koleksi | 100 |
| PK/Carrier RNA | 50 mg/310μg |
| Buffer Larut Protease | 5 ml |
| Buffer VLE | 42 ml |
| Buffer CE | 60 ml |
| RNase Free Water | 15 ml |
Penyimpanan dan Stabilitas
This kit is shipped and stored at room temperature and is valid for 12 bulan.
Ulasan
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