This product provides fast and easy methods for the purification of total DNA for reliable PCR and southern blotting. Total DNA (misalnya, genomik, plasmid) can be purified from bacterial cultures.
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation total DNA from bacterial culture |
| Aplikasi | PCR, noda selatan dan pencernaan enzim, dll.. |
| Teknologi pemurnian | Magnetic beads technology |
| Metode proses | Manual atau otomatis |
| Jenis sampel | Gram negative or positive bacteria |
| Jumlah sampel | Bacterial culture: 0.5-1.5ml |
| Volume elusi | ≥60μl |
| Waktu per lari | ≤60 menit |
Prinsip
- This product utilizes high-binding magnetic particles for purification.
- Samples are lysed and digested using a lysate and Lysozyme.
- DNA is released into the lysate during this process.
- Magnetic particles and binding solution are added, allowing DNA to be adsorbed onto the surface of the particles while impurities such as proteins are not adsorbed.
- The adsorbed particles undergo washing with a washing solution to remove proteins and impurities.
- Ethanol is used for further washing to remove salts.
- Akhirnya, DNA is eluted using Elution Buffer.
Keuntungan
- Cepat: Multiple samples can be extracted within 60 menit (after digestion), making the process time-efficient.
- Kemurnian tinggi: Unique adsorbents ensure the complete removal of inhibitory factors, resulting in high-purity DNA extraction.
- Pemulihan tinggi: DNA can be recovered at the level of picograms (PG), indicating a high level of efficiency in the extraction process.
Isi Paket
| Isi | D513101 | D513102 | D513103 |
| Waktu Pemurnian | 48 Persiapan | 96 Persiapan | 480 Persiapan |
| MagPure Particles | 1.2 ml | 2.5 ml | 11 ml |
| Lysozyme | 60 mg | 120 mg | 600 mg |
| Proteinase K | 24 mg | 50 mg | 220 mg |
| Buffer Larut Protease | 3 ml | 6 ml | 30 ml |
| Penyangga SDS | 1.5 ml | 3 ml | 15 ml |
| Buffer MLA | 30 ml | 60 ml | 300 ml |
| Buffer GW1* | 13 ml | 44 ml | 110 ml |
| Buffer TE | 30 ml | 40 ml | 200 ml |
Penyimpanan dan Stabilitas
- MagPure Particles, Lysozyme, and Proteinase K should be stored at 2-8°C upon arrival.
- Short-term storage (hingga 12 minggu) pada suhu kamar (15-25°C) tidak mempengaruhi kinerja mereka.
- Komponen kit lainnya dapat disimpan kering pada suhu kamar (15-25°C) and remain stable for at least 18 bulan dalam kondisi ini.
Ulasan
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