MagPure Circulating DNA Rich Kit designed for purification of high quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Rich small fragment cfDNA from 0.6ml serum plasma, remove DNA fragments＞500bp |
| Aplikasi | qPCR, NGS, dll.. |
| Teknologi pemurnian | Magnetic beads technology |
| Metode proses | Manual or automatic |
| Jenis sampel | Serum, plasma |
| Jumlah sampel | 0.6ml |
| Volume elusi | |
| Waktu per lari |
Prinsip
This product is based on the purification method of high binding magnetic particles. The sample is lysedand digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, Large DNA(>500bp) will be adsorbed on the surface of magBindparticles. After removal of large size, small size of DNA(<500bp) will be adsorbed on the surface ofMagPure Particle F and impurities such as proteins will be removed without adsorption. The adsorbedparticles were washed with washing solution to remove proteins and impurities, washed with ethanol toremove salts, and finally DNA
Keuntungan
- Economy – less than 50% of the price of Qiagen and other imported products
- Automatic – without labour
Isi Paket
| Isi | 1292750 | 12927200 |
| Waktu Pemurnian | 50 persiapan | 200 persiapan |
| MagPure Particles F | 1.2 ml | 4.5 ml |
| MagBind Particles | 1.2 ml | 4.5 ml |
| Proteinase K | 24 mg | 90 mg |
| Buffer Larut Protease | 1.8 ml | 10 ml |
| Buffer MLB | 30 ml | 120 ml |
| Buffer GDP | 15 ml | 60 ml |
| Buffer MAW1 | 40 ml | 250 ml |
| Buffer MW2* | 20 ml | 50 ml |
| Penyangga Elusi | 15 ml | 60 ml |
Penyimpanan dan Stabilitas
Proteinase K, MagBind Particles and MagPure Particles F should be stored at 2–8°C upon arrival. Namun, penyimpanan jangka pendek (hingga 12 minggu) pada suhu kamar (15-25°C) tidak mempengaruhi kinerja mereka. Komponen kit lainnya dapat disimpan kering pada suhu kamar (15-25°C) dan setidaknya stabil 18 bulan dalam kondisi ini. The entire kit can be stored at 2-8°C, tetapi dalam hal ini buffer harus dilarutkan kembali sebelum digunakan. Pastikan semua buffer berada pada suhu ruangan saat digunakan.
Ulasan
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