Perkenalan
This product is suitable for rapid extraction of DNA from tissue, cells, darah, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Detail
Spesifikasi
Fitur | Spesifikasi |
Fungsi utama | Isolation total DNA from 1-100ul blood, FFPE, tissue and other samples |
Aplikasi | Second generation sequencing, PCR, real timePCR, dll.. |
Metode pemurnian | Monodisperse magnetic beads |
Teknologi pemurnian | Magnetic beads technology |
Metode proses | Manual or automatic |
Jenis sampel | Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells |
Jumlah sampel | Body fluid : 10 – 200μl, Jaringan : <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≤10mg |
Menghasilkan | 10ng – 15μg |
Volume elusi | 80 -100μl |
Waktu per lari |
Prinsip
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Keuntungan
- High binding force – suitable for handling DNA rich samples, such as whole blood, buffy coat, concentrated blood, dll..
- Kemurnian tinggi – carboxyl magnetic beads with weak surface adsorption, getting higher purity
- General – can be used for various clinical samples
Isi Paket
Isi | IVD3101 |
Waktu Pemurnian | 200 |
MagBind Particles | 4.5 ml |
Proteinase K | 90 mg |
Buffer Larut Protease | 10 ml |
Buffer ATL | 60 ml |
Buffer AL | 60 ml |
Buffer BD* | 20 ml |
Buffer BXW1* | 110 ml |
Penyangga Elusi | 30 ml |
Penyimpanan dan Stabilitas
Proteinase K, MagBind Particles should be stored at 2–8°C upon arrival. Namun, short-termstorage (hingga 18 minggu) pada suhu kamar (15–25°C) tidak mempengaruhi kinerja mereka. The remainingkit components can be stored dry at room temperature (15–25°C) dan setidaknya stabil 18 bulan dalam kondisi ini.
Ulasan
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