Perkenalan
- Extracts DNA from various sources: jaringan, cells, darah, saliva, swabs, blood spots, semen, and other clinical samples.
- Fast and easy to use.
- Purified DNA can be used for PCR, qPCR, Southern Blot, viral DNA testing, dan banyak lagi.
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation of total DNA from 1-100ul blood, FFPE, jaringan, and other samples |
| Aplikasi | Second-generation sequencing, PCR, PCR waktu nyata, dll.. |
| Metode pemurnian | Monodisperse magnetic beads |
| Teknologi pemurnian | Magnetic beads technology |
| Metode proses | Manual atau otomatis |
| Jenis sampel | Anticoagulant blood, concentrated blood, buffy coat, lymphocytes, and cultured cells |
| Jumlah sampel | Body fluid : 10 – 200μl, Jaringan : ≤10mg |
| Menghasilkan | 10ng – 15μg |
| Volume elusi | 80 -100μl |
| Waktu per lari |
Prinsip
- Utilizes high-binding magnetic particles for purification, ensuring efficient DNA isolation.
- The sample undergoes lysis and digestion facilitated by lysate and Protease, releasing DNA into the solution.
- Magnetic particles and binding solution are introduced, causing DNA to adhere to particle surfaces while impurities such as proteins are left unbound.
- Wash with a specialized solution to remove proteins and impurities, followed by ethanol wash to eliminate salts.
- DNA is ultimately eluted using an Elution Buffer, providing purified DNA ready for downstream applications.
Advantage
- High binding capability – ideal for processing DNA-rich samples like whole blood, buffy coat, and concentrated blood.
- Exceptional purity – features carboxyl magnetic beads with minimal surface adsorption, ensuring superior purity.
- Versatile – applicable across a wide range of clinical samples, offering general utility.
Isi Paket
| Isi | IVD3101 |
| Waktu Pemurnian | 200 |
| MagBind Particles | 4.5 ml |
| Proteinase K | 90 mg |
| Buffer Larut Protease | 10 ml |
| Buffer ATL | 60 ml |
| Buffer AL | 60 ml |
| Buffer BD* | 20 ml |
| Buffer BXW1* | 110 ml |
| Penyangga Elusi | 30 ml |
Penyimpanan dan Stabilitas
- Proteinase K and MagBind Particles should be stored at 2–8°C upon receipt.
- Short-term storage (hingga 18 minggu) pada suhu kamar (15–25°C) does not compromise their performance.
- The remaining components can be stored dry at room temperature (15–25°C) and remain stable for at least 18 bulan dalam kondisi ini.
