Perkenalan
A highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. This kit can be easily used in manual and automated 96 or 384-well formats.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Recover 100-400μl DNA/ RNA from PCR products / enzymatic reaction solution / or crude DNA / RNA |
| Aplikasi | Automatic sequencing, enzyme digestion, PCR and labeling |
| Teknologi pemurnian | Magnetic beads technology |
| Metode proses | Manual atau otomatis |
| Jenis sampel | Agarose Gel, PCR products, enzymatic reaction solution |
| Jumlah sampel | 100-400μl |
| Recovery | 80% |
| Volume elusi | ≥30μl |
Prinsip
The MagPure method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments 100bp and larger toparamagnetic beads. Excess primers, nucleotides, salts, and enzymes can be removed using a simple washing procedure.
Keuntungan
- High recovery efficiency – hingga 80% DNA recovery
Isi Paket
| Isi | D500301 | D500302 | D500303 |
| Waktu Pemurnian | 50 Persiapan | 500 Persiapan | 5000 Persiapan |
| Buffer AL | 10 ml | 60 ml | 550 ml |
| Buffer BD* | 5 ml | 25 ml | 2 X 100 ml |
| MagPure RNA Particles | 1.2 ml | 12 ml | 120 ml |
| RNase Free Water | 5 ml | 30 ml | 250 ml |
Penyimpanan dan Stabilitas
MagPure RNA Particles should be stored at 2-8°C upon arrival. Namun, penyimpanan jangka pendek (hingga 12 minggu) pada suhu kamar (15-25°C) tidak mempengaruhi kinerjanya. Komponen kit lainnya dapat disimpan kering pada suhu kamar (15-25°C) dan setidaknya stabil 18 bulan dalam kondisi ini. The entire kit can be stored at 2-8°C, tetapi dalam hal ini buffer harus dilarutkan kembali sebelum digunakan. Pastikan semua buffer berada pada suhu ruangan saat digunakan.
Ulasan
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