MagPure FFPE DNA Kit (High Pure)

$149.00$288.00

Pengiriman Rp 45 - Gratis lebih dari USD 300

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Deskripsi

Ikhtisar Produk

  • Suitable for rapid DNA extraction from FFPE sampel.
  • Utilizes two combination methods: High-salt Bind and Alcohol mediated adsorption.
  • High-salt Bind removes pigments or polysaccharides from complex FFPE samples, enhancing nucleic acid purity and preventing blockages in aligent 2100.
  • Alcohol-mediated adsorption improves nucleic acid yield in high-yield samples.

Spesifikasi

FiturSpesifikasi
Fungsi utamaIsolation of high pure total DNA from FFPE using high bind beads
AplikasiPCR and viral DNA detection, dll..
Teknologi pemurnianMagnetic beads technology
Metode prosesManual or automatic
Jenis sampelParaffin-embedded tissue samples
Jumlah sampel1-6 slices of 10-20μm
Volume elusi≥30μl
Waktu per lari≤60 menit

Prinsip

  • Utilizes high-binding magnetic particles for purification.
  • The sample is lysed and digested with lysate and Protease.
  • DNA is released into the lysate during this process.
  • Magnetic particles and binding solution are added, leading to DNA adsorption on particle surfaces.
  • Impurities like proteins are not adsorbed and are removed.
  • Washed with washing solution to remove proteins and impurities.
  • Ethanol wash removes salts.
  • DNA is finally eluted using an Elution Buffer.

Keuntungan

  • Hasil tinggi – most optimal process, recovery up to 90%
  • Economyless than 50% of the price of Qiagen and other imported products
  • Kemurnian tinggi – OD 260/280: 1.7-1.9, OD 260/230: 1.5-2.0

Isi Paket

IsiD632301DD632302D
Waktu Pemurnian48 Persiapan96 Persiapan
MagPure Particles N1.1 ml2.5 ml
RNase A10 mg20 mg
Proteinase K24 mg48 mg
Buffer Larut Protease3 ml6 ml
Buffer DPS60 ml100 ml
Buffer ATL15 ml30 ml
Buffer BST130 ml60 ml
Buffer BW113 ml44 ml
Penyangga Elusi15 ml30 ml

Penyimpanan dan Stabilitas

  • Proteinase K, RNase A, and MagPure Particles N should be stored at 2-8°C initially.
  • Short-term storage at room temperature (15-25°C) for up to 12 weeks doesn’t affect their performance.
  • Remaining kit components can be stored at room temperature and remain stable for at least 18 bulan dalam kondisi ini.

Informasi Tambahan

Berat0.7 kg
ukuran

MagPure FFPE DNA Kit (High Pure) 48 persiapan, MagPure FFPE DNA Kit (High Pure) 96 persiapan

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