Perkenalan
This kit is suitable for high-throughput extraction of total RNA from paraffin embedded tissue samples, including miRNA. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation.The whole extraction process only takes 120 menit. The obtained RNA can be directly used in RT-PCR. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 holeo scillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation total RNA from FFPE jaringan |
| Aplikasi | RT-PCR, cDNA synthesis, second generation sequencing |
| Produk | RNA, miRNA |
| Metode pemurnian | Polydisperse magnetic beads |
| Teknologi pemurnian | Magnetic beads technology |
| Metode proses | Manual or automatic |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
| Jenis sampel | FFPE slice, FFPE embedded tissue |
| Jumlah sampel | ≤6 slices |
Prinsip
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Keuntungan
- Cepat – beberapa sampel dapat diekstraksi 120 minutes by column method
- Aman – no phenol chloroform extraction required
Isi Paket
| Isi | IVD3022 |
| Waktu Pemurnian | 200 Persiapan |
| MagBind Particles | 4.5 ml |
| Proteinase K | 100 mg |
| Buffer Larut Protease | 6 ml |
| DNase I | 4 X 600 μl |
| DNase Buffer | 30 ml |
| Buffer DPS | 200 ml |
| Buffer FRL | 40 ml |
| Buffer AL | 40 ml |
| Buffer MW1* | 110 ml |
| Buffer MW2* | 2 X 50 ml |
| Nuclease Free Water | 30 ml |
Penyimpanan dan Stabilitas
Proteinase K and MagBind Particles should be stored at 2–8°C upon arrival. DNase I should bestored at -20°C. Namun, penyimpanan jangka pendek (DNase I up to 1 minggu, Proteinase K and MagBind Particles up to 8 minggu) pada suhu kamar (15–25°C) tidak mempengaruhi kinerja mereka. The remaining kit components can be stored at room temperature (15–25°C) dan setidaknya stabil 18 months under theseconditions.
Ulasan
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