Perkenalan
This product is suitable for extracting total pathogen RNA from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, dll.. The purified RNA can be used for clinical in vitro detection.
It is special designed for Pathogen Ekstraksi RNA from Sputum samples, can be used in tuberculosis (TB) deteksi.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Main Function | Extract total pathogen RNA from cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant, special design for sputum samples. Used in tuberculosis detection. |
| Aplikasi | RT-PCR，PCR |
| Produk | Pathogen RNA |
| Metode pemurnian | Polydisperse magnetic beads |
| Teknologi pemurnian | Magnetic beads technology |
| Metode proses | Manual or automatic |
| Jenis sampel | cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant, dahak |
| Jumlah sampel | 200-300μl |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
Prinsip
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Keuntungan
- Cepat – beberapa sampel dapat diekstraksi 40 minutes by column method
- High quality – high purity total RNA / DNA can be directly used in various sensitive downstream applications
- Aman – no phenol chloroform extraction required
- Sensitive – DNA/RNA can be recovered at the level of PG
Isi Paket
| Isi | IVD6672C |
| Waktu Pemurnian | 200 Persiapan |
| 2ml Bead Tube | 4 X 50 |
| MagPure Particle | 7.0 ml |
| Proteinase K | 100 mg |
| Buffer Larut Protease | 6 ml |
| DTT （Powder) | 2G |
| Buffer SDS | 15 ml |
| Buffer MLBN | 220 ml |
| DNase I | 4 X 0.6 ml |
| DNase Buffer | 60 ml |
| Buffer MW1* | 53 ml |
| Buffer MW2* | 50 ml |
| Buffer NFW | 30 ml |
Penyimpanan dan Stabilitas
MagPure Particles and Proteinase K, DNase I should be stored at 2–8°C upon arrival. Namun, penyimpanan jangka pendek (hingga 2 minggu) pada suhu kamar (15–25°C) tidak mempengaruhi kinerja mereka. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 bulan dalam kondisi ini.
Ulasan
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