Perkenalan
The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy DNA plasmid from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation up to 15μg endotoxin free plasmid DNA from 1-5ml bacterial culture |
| Aplikasi | Pencernaan enzim, pengurutan, PCR and labeling, dll.. |
| Teknologi pemurnian | Magnetic beads technology |
| Metode proses | Manual or automatic |
| Jenis sampel | Plasmid konvensional, plasmid≤30KB |
| Jumlah sampel | 1-5ml |
| Volume elusi | ≥50μl |
| Waktu per lari | ≤80 minutes |
Prinsip
This product is based on the purification method of high-binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with a washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally, DNA was eluted with an Elution Buffer.
Keuntungan
- Kemurnian tinggi – Plasmid yang dimurnikan dapat langsung digunakan dalam pengurutan, PCR pencernaan enzim, dll..
- Cepat – hanya dibutuhkan 80 minutes to complete the isolation
- Hasil tinggi – up to 15μg plasmid can be bound in one column
- Economic – excellent cost-effectiveness performance
Isi Paket
| Isi | P181402 | P181403 | P181404 |
| Waktu Pemurnian | 100 Persiapan | 500 Persiapan | 5000 Persiapan |
| RNase A | 10 mg | 30 mg | 2 X 160 mg |
| Penyangga P1 | 30 ml | 150 ml | 2 X 800 ml |
| Penyangga P2 | 30 ml | 150 ml | 2 X 800 ml |
| Buffer LEN3 | 20 ml | 80 ml | 800 ml |
| Buffer LN4 | 90 ml | 400 ml | 4 X 980 ml |
| MagPure Particles | 3.5 ml | 17 ml | 3 X 60 ml |
Penyimpanan dan Stabilitas
RNase A and MagPure Particles should be stored at 2–8°C upon arrival. Namun, penyimpanan jangka pendek (hingga 12 minggu) pada suhu kamar (15–25°C) tidak mempengaruhi kinerjanya. Komponen kit lainnya dapat disimpan kering pada suhu kamar (15–25°C) dan setidaknya stabil 18 bulan dalam kondisi ini. Seluruh kit dapat disimpan pada suhu 2–8°C, but in this case, buffers should be redissolved before use. Pastikan semua buffer berada pada suhu ruangan saat digunakan. Jika ada endapan yang terbentuk di buffer, hangatkan pada suhu 37℃ hingga larut. Setelah penambahan RNase A, Buffer P1 stabil untuk 6 months when stored at
Ulasan
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