Perkenalan
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation miRNA from 0.3-0.5ml serum or plasma using magnetic particles |
| Aplikasi | RT-PCR, cDNA synthesis, second generation sequencing |
| Metode pemurnian | Polydisperse magnetic beads |
| Teknologi pemurnian | Magnetic beads technology |
| Metode proses | Manual or automatic |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
| Jenis sampel | Serum, plasma, acellular samples |
| Jumlah sampel | 300μl |
Prinsip
This product is based on the purification method of high binding magnetic particles. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.
Keuntungan
- Aman – no phenol chloroform extraction and no use of Trizol reagent
- Cepat – beberapa sampel dapat diekstraksi 60 minutes by column method
Isi Paket
| Isi | R662801 | R662802 | R662803 |
| Waktu Pemurnian | 48 Persiapan | 96 Persiapan | 5 X 96 Persiapan |
| MagPure Particles N | 1.7 ml | 3.5 ml | 17 ml |
| Buffer CFL | 6 ml | 12 ml | 60 ml |
| Buffer CPL | 1.8 ml | 3.5 ml | 20 ml |
| Buffer MGW1* | 30 ml | 60 ml | 250 ml |
| Buffer MW2* | 12 ml | 25 ml | 100 ml |
| RNase Free Water | 10 ml | 20 ml | 60 ml |
Penyimpanan dan Stabilitas
MagPure Particle N should be stored at 2–8°C upon arrival. Namun, penyimpanan jangka pendek (hingga 8 minggu) pada suhu kamar (15–25°C) tidak mempengaruhi kinerja mereka. The remaining kit components can be stored at room temperature (15–25°C) dan setidaknya stabil 18 bulan dalam kondisi ini.
Ulasan
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