This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 menit. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation total DNA from 250-500mg soil sample |
| Aplikasi | PCR, noda selatan dan pencernaan enzim, dll.. |
| Teknologi pemurnian | Magnetic beads technology |
| Metode proses | Manual or automatic |
| Jenis sampel | Soil |
| Jumlah sampel | 250-500mg |
| Volume elusi | ≥50μl |
| Waktu per lari | ≤70 minutes |
Prinsip
This product is based on the purification method of high binding magnetic particles. Soil sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, protein, polysaccharides, and other contaminants are removed using our propietary Absorber Solution. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing buffer to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Keuntungan
- Konsentrasi tinggi – maximum extraction of total DNA from soil samples
- Kemurnian tinggi – purified DNA can be directly used in various downstream applications
Isi Paket
| Isi | D635601 | D635602 | D635603 |
| Waktu Pemurnian | 48 Persiapan | 96 Persiapan | 5 X 96 Persiapan |
| MagPure Particles | 2.5 ml | 5 ml | 22 ml |
| 2ml Bead Tubes | 48 | 96 | 400 |
| Buffer SOL | 60 ml | 100 ml | 500 ml |
| Buffer SDS | 6 ml | 10 ml | 50 ml |
| Reagen DX | 1 ml | 1 ml | 5 ml |
| Penyangga PS | 10 ml | 20 ml | 90 ml |
| Absorber Solution | 10 ml | 20 ml | 90 ml |
| Buffer GDP | 70 ml | 150 ml | 2 X 350 ml |
| Buffer GW1* | 22 ml | 44 ml | 220 ml |
| Penyangga Elusi | 20 ml | 20 ml | 60 ml |
Penyimpanan dan Stabilitas
MagPure Particles and Absorber Solution should be stored at 2-8°C upon arrival. Namun, penyimpanan jangka pendek (hingga 12 minggu) pada suhu kamar (15-25°C) tidak mempengaruhi kinerja mereka. Komponen kit lainnya dapat disimpan kering pada suhu kamar (15-25°C) dan setidaknya stabil 18 bulan dalam kondisi ini.
Data Eksperimen
Ulasan
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