Perkenalan
This product is suitable for rapid extraction of DNA from tissue, cells, darah, saliva, swabs, blood spots, semen, and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA, and so on.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation of total DNA from blood, buffy coat, jaringan, and other samples |
| Aplikasi | Second-generation sequencing, PCR, PCR waktu nyata, dll.. |
| Metode pemurnian | Polydisperse magnetic beads |
| Teknologi pemurnian | Magnetic beads technology |
| Metode proses | Manual or automatic |
| Jenis sampel | Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells |
| Jumlah sampel | Seluruh darah :< 200μl; Saliva / mengusap:< 400μl; Jaringan :< 20mg |
| Menghasilkan | 0.1 – 50μg |
| Volume elusi | |
| Waktu per lari |
Prinsip
This product is based on the purification method of high-binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with a washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally, DNA was eluted with an Elution Buffer.
Keuntungan
- Kemurnian tinggi – OD 260 / 280: 1.7- 1.9, OD 260 / 230: 1.5 – 2.0
- Economy – less than 50% of the price of Qiagen and other imported products
- Hasil tinggi – most optimal process, ensuring the recovery of up to 90%
- Strong processing ability – samples including animal blood, cultured cells, animal tissues, dll..
Isi Paket
| Isi | IVD3102 |
| Waktu Pemurnian | 200 |
| MagPure Particles | 5 ml |
| Proteinase K | 100 mg |
| Buffer Larut Protease | 10 ml |
| Rnase A | 40 mg |
| Buffer ATL | 60 ml |
| Buffer AL | 60 ml |
| Buffer BD* | 20 ml |
| Buffer BW1* | 110 ml |
| Penyangga Elusi | 30 ml |
Penyimpanan dan Stabilitas
Proteinase K, RNase A, MagPure Particles should be stored at 2-8°C upon arrival. Namun, penyimpanan jangka pendek (hingga 24 minggu) pada suhu kamar (15-25°C) tidak mempengaruhi kinerja mereka. The remaining kit components can be stored at room temperature (15-25°C) dan setidaknya stabil 18 bulan dalam kondisi ini.
Data Eksperimen
Ulasan
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