Perkenalan
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | IVD5412 precast kit for kingfisher Flex |
| Aplikasi | RT-PCR，PCR，NGS |
| Produk | Viral DNA / RNA, body cell DNA / RNA |
| Metode pemurnian | Polydisperse magnetic beads |
| Teknologi pemurnian | Magnetic beads technolog |
| Metode proses | Manual or automatic |
| Jenis sampel | |
| Jumlah sampel | 200μl |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
Prinsip
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.
Keuntungan
Isi Paket
| Cat.No | Reagen | IVD5412-F-96 |
| PK/Carrier RNA | 1X 24 mg/Bottle | |
| Protease Dissolve Buffer Blue | 1.8 ml/Bottle | |
| Tip | 1 | |
| Sample Plate (DW Plate) | 500µl Buffer MLB/Well | 1 |
| Wash 1 Plate (DW Plate) | 500µl Buffer MW1/Well | 1 |
| Wash 2 Plate (DW Plate) | 500µl Buffer CW/Well | 1 |
| Beads Plate (DW Plate) | 500µl CW & 20µl MPN/Well | 1 |
| Elute Plate (KF Plate) | 90µl NFW/Well | 1 |
Penyimpanan dan Stabilitas
This kit is shipped and stored at room temperature and is valid for 12 bulan.
Ulasan
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