instruksi:
- Sample Collection:
- Collect 0.1-1ml blood sample.
- Persiapan Sampel:
- Menambahkan 2 ke 3 times the volume of 1x red blood cell solution to the blood sample.
- Mix thoroughly by inverting.
- Sentrifugasi di 12,000 rpm untuk 1 menit.
- Carefully aspirate the supernatant. The precipitate should ideally be white or light red.
- Menambahkan 400 μl reagent buffer I and 10 μl epoxy resin K (10 mg/ml) to the precipitate.
- Vortex and mix for 15 detik, then let it sit for 2 menit.
- Special Case: Low-level Organisms:
- If processing blood from low-level organisms (misalnya, unggas, birds) containing cell nuclei:
- Add 1x red blood cell solution and directly add 200 μl of reagent buffer I (total volume not exceeding 500 μl).
- Let it sit for 2 menit.
- Digestion:
- Menambahkan 10 μl epoxy resin K (10 mg/ml) to the mixture.
- Invert and mix, then digest at 65°C for 10 menit.
- Selama periode ini, balikkan dan campur 6-7 times to ensure complete digestion.
- Transfer to Reagent Plate:
- Add the solution from the previous step to the 96-well reagent plate.
- Follow specific steps outlined in the automatic extraction protocol for further processing.
