Serum Ferri Ion Content Assay Kit
Catatan: Ambil dua atau tiga sampel berbeda untuk prediksi sebelum pengujian.
Peralatan Operasi: Spektrofotometer
Cat Number: BC1730
Ukuran: 50T/48S
Komponen:
Reagen I: Bedak×2, storage at 4℃. Menambahkan 10 mL distilled water before use.
Reagent Ⅱ: Bedak×2, storage at 4℃. Menambahkan 313 μL glacial acetic acid and 10 mL distilled water before use. Standard Solution: Cairan 3 mL×1, 1000 μmol/L Fe3+ standard solution, storage at 4℃. Add distilled water dilute 8 times to form a standard solution of 125 μmol/L before use.
Deskripsi Produk:
Serum iron is the iron bound with transferrin in blood, which is often used to distinguish non-iron deficiency anemia and iron-deficiency anemia.
Fe3+ is reduced by sodium sulfite to Fe2+, which reacts with 2,2-dipyridine-bipyridine, have an absorption peak at 520 nm. According measure absorbance at 520 nm can reflect serum iron concentration.
Reagen dan Peralatan Diperlukan tetapi Tidak Disediakan.
Spektrofotometer, alat sentrifugal, glacial acetic acid, adjusted transferpettor, 1 mL kuvet kaca, chloroform and distilled water.
Prosedur:
- Preheat the spectrophotometer for 30 menit, sesuaikan panjang gelombang ke 520 nm, atur nol dengan sulingan
- Dilute standard solution to 125 μmol/L with distilled
- Tambahkan reagen dengan daftar berikut:
| Nama Reagen (μL) | Tabung kosong (AB) | Tabung reaksi (PADA) | Standard tube (SEBAGAI) |
| Air sulingan | 400 | ||
| Solusi standar (125 μmol/L) | 400 | ||
| Serum(plasma) | 400 | ||
| Reagen I | 400 | 400 | 400 |
| Reagen II | 400 | 400 | 400 |
| Aduk rata, incubate in boiling water bath for 5 menit, cooling liquid. Menambahkan 200 μL chloroform (required but not provided). Aduk rata, room temperature, 10000 rpm centrifuge for 10 menit. Mengambil 700 μL supernatant to 1 mL kuvet kaca. Measure absorbance at 520 nm. Recorded as AB, PADA, SEBAGAI. | |||
Perhitungan
Serum iron (μmol/L)=[Cs×(AT-AB)÷(As-AB)]= 125×(AT-AB)÷(As-AB)
Cs: Fe3+ standard solution, 125 μmol/L.
Catatan:
- There is less iron in the serum, so the vessels (EP tubes) should be noted to avoid iron contamination.
- Reagent I and Reagent II are unstable. It needs to be prepared when the solution will be used, and the newly prepared reagent can only be used on the same day.
- When At- Ac＞5, please dilute the Serum to appropriate concentration with distilled water.
Technical Specifications：
Minimum Detection Limit：3.212 μmol/mL
Linear Range：3.9-250 μmol/mL
Produk-produk terkait：
BC2860/BC2865 Serum Total Iron Binding Capacity(TIBC) Perangkat Pengujian
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Ulasan
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