This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of tissue and culture cells. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation total DNA from tissue using economic salt out method |
| Aplikasi | PCR, enzyme digestion, Southern hybridization,dll.. |
| Teknologi pemurnian | Salting out method |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Animal tissue |
| Jumlah sampel | Unlimited |
| Volume elusi | ≥100μl |
| Waktu per lari | Variation with sample amount |
Principles
Cells are lysed with ananionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Akhirnya, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260/A280 ratio between 1.7 Dan 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2-8°C, -20°C, or -80°C.
Keuntungan
- Aman – without phenol chloroform extraction
- Environment friendly – the reagents used are safe, non-toxic and without pollution
- High molecular weight – the molecular weight of genomic DNA is about 50-150kb
- Kemurnian tinggi – the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
- Unlimited sample size – solution type operation, sample volume can be adjusted at will
- Cost performance – the most economical nucleic acid extraction technology
Isi Paket
| Isi | D331201 | D331202 | D331203 |
| Waktu Pemurnian | 1 G | 5 G | 50 G |
| Proteinase K | 330 μl | 1.8 ml | 18 ml |
| Buffer WTL | 33 ml | 160 ml | 2 X 800 ml |
| Buffer PPS | 12 ml | 55 ml | 500 ml |
| RNase Solution | 330 μl | 1.8 ml | 18 ml |
| Buffer TE | 12 ml | 60 ml | 200 ml |
Penyimpanan dan Stabilitas
RNase Solution should be stored at 2-8°C upon arrival. Namun, penyimpanan jangka pendek (hingga 24 minggu) pada suhu kamar (15-25°C) tidak mempengaruhi kinerja mereka. Komponen kit lainnya dapat disimpan kering pada suhu kamar (15-25°C) dan setidaknya stabil 18 bulan dalam kondisi ini. The entire kit can be stored at 2-8°C, tetapi dalam hal ini buffer harus dilarutkan kembali sebelum digunakan. Pastikan semua buffer berada pada suhu ruangan saat digunakan.
Ulasan
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