This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, plasma, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative PCR, liquid or solid phase chip analysis, hybridization, and SNP detection.
HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.
Dettagli
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation both Circulating DNA/RNA (include miRNA) from 1-5 ml serum and plasma |
| Applicazioni | qPCR / RT-PCR, liquid or solid-phasechip analysis, hybridization and SNP detection |
| Metodo di purificazione | Midi colonna rotante |
| Tecnologia di purificazione | Tecnologia della silice, DNA filtration technology |
| Metodo di processo | Manuale (centrifugazione o vuoto) |
| Tipo di campione | siero, plasma, and other cell-free liquid samples |
| Importo del campione | 1-5 ml |
| Volume di eluizione | ≥20μl |
| Tempo per corsa | ≤100 minutes |
| Volume di trasporto del liquido per colonna | 4 ml |
| Resa legante della colonna | 1 mg |
Principio
This kit is based on silica gel column technology. Serum or other liquid samples are lysed and digested in buffer CFL. After adding buffer CFP, the protein is removed by centrifugation to obtain the supernatant. Isopropanol is added to precipitate the total nucleic acid and transferred to the column for filtration. DNA / RNA is adsorbed on the membrane of the column, while the protein is not adsorbed and removed with the filtrate.The column is washed with buffer MGW1 to remove protein and other impurities, and then washed with buffer RW2 to remove salt. Finalmente, DNA / RNA is eluted by low salt buffer. The eluted DNA / RNA can be directly used for quantitative PCR/ RT-PCR, liquid or solid-phase chip analysis, hybridization and SNP detection.
Vantaggi
- High yield – most optimized process to obtain maximum free RNA and small RNA
- High concentration – low elution volume (>20ml) to ensure nucleic acid concentration
- Purezza elevata – low alcohol combination, completely remove inhibitor and protein pollution
- Recupero elevato – silica gel column technology can recover nucleic acid molecules at the level of PG
- Large volume – 1-2ml serum and plasma samples can be processed at one time
Contenuto del kit
| Contenuti | R431602 | D431603 |
| Tempi di purificazione | 50 Preparativi | 250 Preparativi |
| Microcolonne di RNA HiPure | 50 | 5 X 50 |
| HiPure Viral Midi Columns | 50 | 5 X 50 |
| 15 ml Provette di raccolta | 50 | 5 X 50 |
| 2ml Provette di raccolta | 50 | 5 X 50 |
| Buffer CFL | 150 ml | 2 X 375 ml |
| Buffer CFP | 30 ml | 150 ml |
| Buffer MGW1* | 100 ml | 2 X 250 ml |
| Buffer RW2* | 2 X 50 ml | 5 X 100 ml |
| Acqua libera da RNasi | 10 ml | 50 ml |
Conservazione e stabilità
I componenti del kit possono essere conservati a temperatura ambiente (15–25°C) e sono stabili almeno per 18 mesi in queste condizioni.
Recensioni
Non ci sono ancora recensioni.