The HiPure Fruit RNA Mini Kit provides fast purification of high-quality RNA from fruits, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100µg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA(mRNA) purificazione, nuclease protection and in vitro translation.
Dettagli
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolamento dell'RNA totale da <150 mg fruit or seed using chloroform and Plantzol reagent |
| Applicazioni | RT-PCR, qPCR, Northern hybridization, Poly A purification, nucleic acid protection and in vitro translation translation |
| Metodo di purificazione | Mini colonna rotante |
| Tecnologia di purificazione | Tecnologia della silice, DNA filtration technology |
| Metodo di processo | Manuale (centrifugazione o vuoto) |
| Tipo di campione | Simple plant, petal, peel, seed |
| Importo del campione | 50-150mg |
| Volume di eluizione | ≥300μl |
| Tempo per corsa | ≤15 minutes（1-24 samples） |
| Volume di trasporto del liquido per colonna | 800ml |
| Resa legante della colonna | 100mg |
Principio
The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 15 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 300µl water using the Kit.
Vantaggi
- Efficient DNA removal – one step Estrazione dell'RNA can effectively remove genomic DNA
- Alta qualità – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Veloce – possono essere estratti diversi campioni 15 minutes by column method
- Wide range – samples including animals, impianti, batteri, cellule, eccetera
- High output – large sample amount, and the yield can be up to 200μg
Contenuto del kit
| Contenuti | R401402 | D401403 |
| Tempi di purificazione | 50 Preparativi | 250 Preparativi |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Provette di raccolta | 50 | 250 |
| PlantZol Reagent | 60 ml | 200 ml |
| Buffer GXP | 30 ml | 150 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2* | 20 ml | 2 X 50 ml |
| Acqua libera da RNasi | 10 ml | 30 ml |
Conservazione e stabilità
Il Kit può essere conservato asciutto a temperatura ambiente (15–25°C) e sono stabili almeno per 18 mesi in queste condizioni. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
Recensioni
Non ci sono ancora recensioni.