HiPure HP Plant RNA Mini Kit combines guanidina isothiocyanate lysing and silica gel membrane purification technology to simplify total Estrazione dell'RNA. Ultracentrifugation in CsCl purification and LiCl / ethanol precipitation are not required. The kit uses DNase digestion to completely remove DNA. It is suitable for extracting up to 100μg of total RNA (including miRNA) from plant samples less than 200mg. Several samples can be extracted within 40 minuti. The purified RNA can be directly used for RT-PCR, fluorescent quantitative RT-PCR, Northern hybridization, second generation sequencing, eccetera.
Dettagli
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation total RNA (include miRNA) from <200mg difficult-to-extract plant samples (use low toxicity chloroform substitutes) |
| Applicazioni | RT-PCR, qPCR, Northern hybridization, second generation sequencing, protezione degli acidi nucleici, in vitro translation |
| Metodo di purificazione | Mini colonna rotante |
| Tecnologia di purificazione | Tecnologia della silice |
| Metodo di processo | Manuale (centrifugazione o vuoto) |
| Tipo di campione | Hard-to-extraction plant samples such as fruit and seed, grape leaves, tea |
| Importo del campione | ≤200 mg |
| Volume di eluizione | ≥30μl |
| Tempo per corsa | 1-24 samples within 30 minuti |
| Volume di trasporto del liquido per colonna | 800ml |
| Resa legante della colonna | 100mg |
Principio
This kit uses glass fiber filter membrane purification technique, and only requires simple combination-washing-elution steps. The sample is lysed and homogenized in the solution containing guanidine salt, ethanol is added to provide appropriate binding conditions, and transferred to the purification column for centrifugation. Up to 100µg of RNA can be selectively bound to the membrane, pollutants are efficiently washed off after three times of washing, and finally the purified RNA is eluted by RNase Free Water.
Vantaggi
- Completely remove DNA by using of DNase
- Alta qualità – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Veloce – possono essere estratti diversi campioni 40 minutes by column method
- Sensibile – RNA can be recovered at the level of PG
- Broad spectrum – various types of plant samples can be processed by diversity of operating procedures
Contenuto del kit
| Contenuti | R416502 | D416503 |
| Tempi di purificazione | 50 Preparativi | 250 Preparativi |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Provette di raccolta | 100 | 250 |
| DNasi I | 600 ml | 5 X 600 ml |
| DNase Buffer | 6 ml | 30 ml |
| Buffer PAL | 60 ml | 270 ml |
| Buffer GXP2* | 20 ml | 100 ml |
| Buffer BDP | 60 ml | 270 ml |
| Buffer RW1 | 50 ml | 250 ml |
| Buffer RW2* | 20 ml | 2 X 50 ml |
| Acqua libera da RNasi | 10 ml | 30 ml |
Conservazione e stabilità
DNase I should be stored at -20-8°C upon arrival. I restanti componenti del kit possono essere conservati a temperatura ambiente (15-25°C) e sono stabili almeno per 18 mesi in queste condizioni. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Assicurarsi che tutti i tamponi siano a temperatura ambiente quando utilizzati.
Recensioni
Non ci sono ancora recensioni.