HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minuti. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
Dettagli
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation total DNA from <10 mg insect tissue |
| Applicazioni | PCR, southern bolt and virus detection, eccetera |
| Metodo di purificazione | Midi colonna rotante |
| Tecnologia di purificazione | Tecnologia della silice |
| Metodo di processo | Manuale (centrifugazione o vuoto) |
| Tipo di campione | Insect tissue samples |
| Importo del campione | <10 mg |
| Volume di eluizione | ≥15μl |
| Tempo per corsa | ≤30 minutes |
| Volume di trasporto del liquido per colonna | 800ml |
| Resa legante della colonna | 100μg |
Principles
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, Il DNA viene rilasciato nel lisato. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Vantaggi
- High purity DNA – can be used in sensitive downstream applications such as multiplex and quantitative pcr
- Veloce – several samples can be extracted in less than 30 minuti
- Good repeatability – suitable for extracting high-yield DNA from insect tissue samples
- Sicuro – without phenol chloroform extraction and alcohol precipitation
Contenuto del kit
| Contenuti | D312902 | D312903 |
| Tempi di purificazione | 50 | 250 |
| HiPure DNA Mini Columns I | 50 | 250 |
| 2ml Provette di raccolta | 50 | 250 |
| Buffer ITL | 30 ml | 120 ml |
| Buffer IL* | 30 ml | 120 ml |
| Buffer GW1* | 22 ml | 110 ml |
| Buffer GW2* | 20 ml | 2 X 50 ml |
| Proteinasi K | 24 mg | 120 mg |
| Tampone di dissoluzione della proteasi | 1.8 ml | 15 ml |
| Buffer AE | 15 ml | 60 ml |
Conservazione e stabilità
Proteinase K should be stored at 2-8°C upon arrival. Tuttavia, conservazione a breve termine (fino a 12 settimane) a temperatura ambiente (15-25°C) non influisce sulle loro prestazioni. I restanti componenti del kit possono essere conservati a temperatura ambiente (15-25°C) e sono stabili almeno per 18 mesi in queste condizioni. L'intero kit può essere conservato a 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.
Recensioni
Non ci sono ancora recensioni.