introduzione
- Veloce, Simple, and Cost-Effective: Offers a rapid, easy-to-use, and economical method for plasmid DNA miniprep suitable for routine molecular biology applications.
- Silica Membrane Technology: Utilizes silica membrane technology, eliminating the need for cumbersome steps associated with loose resins or slurries, streamlining the purification process.
- Immediate Usability: Purified plasmid DNA is immediately ready for downstream applications, saving time and effort.
- No Phenol Extraction or Ethanol Precipitation: Bypasses the need for phenol extraction and ethanol precipitation, simplifying the workflow and reducing hands-on time.
- High-Quality DNA: Yields high-quality plasmid DNA eluted in a small volume of Tris buffer or water, ensuring optimal performance in downstream applications.
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation of up to 35μg plasmid DNA from 1-5ml bacterial culture |
| Applicazioni | Enzyme digestion, sequenziamento, PCR, cloning, eccetera. |
| Metodo di purificazione | Mini colonna rotante |
| Tecnologia di purificazione | Tecnologia della silice |
| Metodo di processo | Manuale (centrifugazione o vuoto) |
| Tipo di campione | Conventional plasmid, plasmid less than 30KB |
| Importo del campione | High copy plasmid: 1-5ml culture medium Low copy number plasmid: 5-10ml culture medium |
| Yield | 5-35mg |
| Volume di eluizione | ≥30μl |
| Tempo per corsa | Complete 1-24 samples in 30 minuti |
| Volume di trasporto del liquido per colonna | 800ml |
| Resa legante della colonna | 35mg |
Principio
- Alkaline Lysis: Begins with the alkaline lysis of bacterial cells, which breaks down the cell membrane and releases plasmid DNA into the lysate.
- Silica-Based DNA Binding: The released DNA is adsorbed onto the silica membrane in the presence of high salt, facilitating efficient binding of DNA to the membrane surface.
- Three Basic Steps: The procedure involves three main steps: (1) Preparation and clearing of the bacterial lysate using an alkaline method, (2) Transfer of the cleared lysate to a column for DNA binding, E (3) Washing to remove proteins and other impurities.
- Silica Membrane: Utilizes a unique silica membrane in the kit, which completely replaces traditional glass or silica slurries typically used in plasmid DNA minipreps.
- Elution with Low-Salt Buffer: After washing to remove impurities, acido nucleico, including plasmid DNA, is eluted from the silica membrane using a low-salt buffer containing 10mM Tris, pH 9.0, and 0.5mM EDTA.
Vantaggi
- Purezza elevata – purified plasmid can be directly used in sequencing, enzyme digestion PCR, eccetera.
- Veloce – it takes only 1 minute to obtain supernatant by optimized solution (10 minutes for other brands)
- High yield – up to 35µg plasmid can be bound in one column
Contenuto del kit
| Contenuti | P100102 | P100103 |
| Tempi di purificazione | 100 Preparativi | 250 Preparativi |
| RNasi A | 5 mg | 10 mg |
| Buffer P1 | 30 ml | 80 ml |
| Buffer P2 | 30 ml | 80 ml |
| Buffer P3 | 40 ml | 100 ml |
| Buffer PW1 | 60 ml | 140 ml |
| Buffer PW2* | 20 ml | 50 ml |
| Tampone di eluizione | 15 ml | 30 ml |
| HiPure DNA Mini Columns II | 100 | 250 |
| 2 ml Provette di raccolta | 100 | 250 |
Conservazione e stabilità
- Condizioni di archiviazione: Kit components can be stored dry at room temperature (15-25°C) and remain stable for a minimum of 18 mesi in queste condizioni.
- Buffer Precipitates: In case of any precipitates forming in the buffers, warm them at 37°C to dissolve effectively.
- Buffer P1 Stability: After the addition of RNase A, Buffer P1 remains stable for up to 6 months when stored at 2-8°C.
Dati dell'esperimento
Guida all'acquisto
| Nome | GATTO N | Importo del campione | Column type | Volume di eluizione | Yield | Tempo per corsa | Centrifuge requirements |
| Mini kit plasmide HiPure | P1001 | 1-5ml | 1.5ml column | 30-50ml | 5–35µg/5ml | ≤30 minutes | small centrifuge |
| HiPure Plasmid Mini Kit II | P1002 | 5-15ml | 1.5ml column | 75-100ml | 20-80µg/15ml | ≤30 minutes | small centrifuge |
| HiPure Plasmid Plus 96 Kit | P1006 | 1-1.5ml | 96 bene piatto | 70-150ml | 1-15μg/1ml | ≤60 minuti | 96-well plate centrifuge |
| HiPure Fastfilter Plasmid Midi Kit | P1013 | 30-50ml | 15ml column | 0.3-0.8ml | 50–250µg/50ml | ≤60 minuti | 15ml centrifuge |
| HiPure Fastfilter Plasmid Maxi Kit | P1014 | 100-200ml | 50ml column | 0.5-1.5ml | 0.4–1mg/200ml | ≤60 minuti | 50ml centrifuge |
Recensioni
Non ci sono ancora recensioni.